Local transposition of a hobo element within the decapentaplegic locus of Drosophila

Stuart Newfeld, Norma T. Takaesu

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

We have efficiently mobilized a phenotypically silent hobo transgene inserted within the cis-regulatory heldout region of the decapentaplegic (dpp) locus in Drosophila melanogaster. The goal of our experiment was to identify germline transmission of a local transposition event within the dpp locus that meets two specific criteria. First, excision of the hobo construct does not generate an adult mutant phenotype, suggesting minimal alteration to the original site of insertion. Second, we required a new insertion of the hobo transgene into the Haploinsufficient region of the locus ~25 kb away. Genetic and molecular criteria are used to evaluate candidate germlines. In a pilot study, this local transposition event occurred independently in two individuals. Both of the transposition events appear to be new insertions into the dpp transcription unit. One insertion is between the two protein- coding exons, and the other is in the 3'-untranslated region of exon three. Strains carrying these insertions are valuable new reagents for the analysis of dpp function and molecular evolution. These results further support the use of the hobo system as an important tool in Drosophila genetics.

Original languageEnglish (US)
Pages (from-to)177-187
Number of pages11
JournalGenetics
Volume151
Issue number1
StatePublished - Jan 1999

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Transgenes
Drosophila
Exons
Molecular Evolution
Nucleic Acid Regulatory Sequences
3' Untranslated Regions
Drosophila melanogaster
Molecular Biology
Phenotype
Proteins

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Local transposition of a hobo element within the decapentaplegic locus of Drosophila. / Newfeld, Stuart; Takaesu, Norma T.

In: Genetics, Vol. 151, No. 1, 01.1999, p. 177-187.

Research output: Contribution to journalArticle

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