Liquid crystal thermography of an on-chip polymerase chain reaction micro-thermocycler

Tracy Helen Fung, Shih-Hui Chao, Joseph E. Peach, Deirdre Meldrum

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

Microscale liquid crystal thermography is a technique to measure temperature distribution of microfabricated devices in real-time. This method utilizes a microscope to image the color map of a layer of temperature-sensitive encapsulated thermochromic liquid crystals (TLC) coated on a microfabricated device. This paper describes the TLC coating process on microscale devices, the characteristics of colorimetric hysteresis, and the calibration of temperature measurements. The calibrated measurements have been applied for characterization of an on-chip polymerase chain reaction (PCR) microscale thermocycler where precise and dynamic temperature control is essential for efficient DNA amplification. Tests on the micro-thermocycler were done around the ranges centered at 30°C and 95°C. The results illustrate the effects on the temperature distribution due to micro-thermocycler geometry, and provide important insight for micro-thermocycler design.

Original languageEnglish (US)
Title of host publicationProceedings of the 4th International Conference on Nanochannels, Microchannels and Minichannels, ICNMM2006
Pages1039-1044
Number of pages6
Volume2006 B
StatePublished - 2006
Externally publishedYes
Event4th International Conference on Nanochannels, Microchannels and Minichannels, ICNMM2006 - Limerick, Ireland
Duration: Jun 19 2006Jun 21 2006

Other

Other4th International Conference on Nanochannels, Microchannels and Minichannels, ICNMM2006
CountryIreland
CityLimerick
Period6/19/066/21/06

Fingerprint

Polymerase chain reaction
Liquid crystals
Temperature distribution
Temperature control
Temperature measurement
Amplification
Hysteresis
Microscopes
DNA
Calibration
Color
Coatings
Geometry
Temperature

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Fung, T. H., Chao, S-H., Peach, J. E., & Meldrum, D. (2006). Liquid crystal thermography of an on-chip polymerase chain reaction micro-thermocycler. In Proceedings of the 4th International Conference on Nanochannels, Microchannels and Minichannels, ICNMM2006 (Vol. 2006 B, pp. 1039-1044)

Liquid crystal thermography of an on-chip polymerase chain reaction micro-thermocycler. / Fung, Tracy Helen; Chao, Shih-Hui; Peach, Joseph E.; Meldrum, Deirdre.

Proceedings of the 4th International Conference on Nanochannels, Microchannels and Minichannels, ICNMM2006. Vol. 2006 B 2006. p. 1039-1044.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Fung, TH, Chao, S-H, Peach, JE & Meldrum, D 2006, Liquid crystal thermography of an on-chip polymerase chain reaction micro-thermocycler. in Proceedings of the 4th International Conference on Nanochannels, Microchannels and Minichannels, ICNMM2006. vol. 2006 B, pp. 1039-1044, 4th International Conference on Nanochannels, Microchannels and Minichannels, ICNMM2006, Limerick, Ireland, 6/19/06.
Fung TH, Chao S-H, Peach JE, Meldrum D. Liquid crystal thermography of an on-chip polymerase chain reaction micro-thermocycler. In Proceedings of the 4th International Conference on Nanochannels, Microchannels and Minichannels, ICNMM2006. Vol. 2006 B. 2006. p. 1039-1044
Fung, Tracy Helen ; Chao, Shih-Hui ; Peach, Joseph E. ; Meldrum, Deirdre. / Liquid crystal thermography of an on-chip polymerase chain reaction micro-thermocycler. Proceedings of the 4th International Conference on Nanochannels, Microchannels and Minichannels, ICNMM2006. Vol. 2006 B 2006. pp. 1039-1044
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