Lipidic cubic phase serial millisecond crystallography using synchrotron radiation

Przemyslaw Nogly, Daniel James, Dingjie Wang, Thomas A. White, Nadia Zatsepin, Anastasya Shilova, Garrett Nelson, Haiguang Liu, Linda Johansson, Michael Heymann, Kathrin Jaeger, Markus Metz, Cecilia Wickstrand, Wenting Wu, Petra Båth, Peter Berntsen, Dominik Oberthuer, Valerie Panneels, Vadim Cherezov, Henry Chapman & 7 others Gebhard Schertler, Richard Neutze, John Spence, Isabel Moraes, Manfred Burghammer, Joerg Standfuss, Uwe Weierstall

Research output: Contribution to journalArticle

91 Citations (Scopus)

Abstract

Lipidic cubic phases (LCPs) have emerged as successful matrixes for the crystallization of membrane proteins. Moreover, the viscous LCP also provides a highly effective delivery medium for serial femtosecond crystallography (SFX) at X-ray free-electron lasers (XFELs). Here, the adaptation of this technology to perform serial millisecond crystallography (SMX) at more widely available synchrotron microfocus beamlines is described. Compared with conventional microcrystallography, LCP-SMX eliminates the need for difficult handling of individual crystals and allows for data collection at room temperature. The technology is demonstrated by solving a structure of the light-driven proton-pump bacteriorhodopsin (bR) at a resolution of 2.4±Å. The room-temperature structure of bR is very similar to previous cryogenic structures but shows small yet distinct differences in the retinal ligand and proton-transfer pathway.

Original languageEnglish (US)
Pages (from-to)168-176
Number of pages9
JournalIUCrJ
Volume2
DOIs
StatePublished - Feb 26 2015

Fingerprint

Bacteriorhodopsins
Crystallography
Synchrotrons
Synchrotron radiation
crystallography
synchrotron radiation
Radiation
X ray lasers
Technology
Proton Pumps
Temperature
Proton transfer
protons
Free electron lasers
room temperature
Crystallization
free electron lasers
Cryogenics
cryogenics
Protons

Keywords

  • bacteriorhodopsin
  • lipidic cubic phases
  • protein crystallography
  • XFEL

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Biochemistry

Cite this

Lipidic cubic phase serial millisecond crystallography using synchrotron radiation. / Nogly, Przemyslaw; James, Daniel; Wang, Dingjie; White, Thomas A.; Zatsepin, Nadia; Shilova, Anastasya; Nelson, Garrett; Liu, Haiguang; Johansson, Linda; Heymann, Michael; Jaeger, Kathrin; Metz, Markus; Wickstrand, Cecilia; Wu, Wenting; Båth, Petra; Berntsen, Peter; Oberthuer, Dominik; Panneels, Valerie; Cherezov, Vadim; Chapman, Henry; Schertler, Gebhard; Neutze, Richard; Spence, John; Moraes, Isabel; Burghammer, Manfred; Standfuss, Joerg; Weierstall, Uwe.

In: IUCrJ, Vol. 2, 26.02.2015, p. 168-176.

Research output: Contribution to journalArticle

Nogly, P, James, D, Wang, D, White, TA, Zatsepin, N, Shilova, A, Nelson, G, Liu, H, Johansson, L, Heymann, M, Jaeger, K, Metz, M, Wickstrand, C, Wu, W, Båth, P, Berntsen, P, Oberthuer, D, Panneels, V, Cherezov, V, Chapman, H, Schertler, G, Neutze, R, Spence, J, Moraes, I, Burghammer, M, Standfuss, J & Weierstall, U 2015, 'Lipidic cubic phase serial millisecond crystallography using synchrotron radiation', IUCrJ, vol. 2, pp. 168-176. https://doi.org/10.1107/S2052252514026487
Nogly P, James D, Wang D, White TA, Zatsepin N, Shilova A et al. Lipidic cubic phase serial millisecond crystallography using synchrotron radiation. IUCrJ. 2015 Feb 26;2:168-176. https://doi.org/10.1107/S2052252514026487
Nogly, Przemyslaw ; James, Daniel ; Wang, Dingjie ; White, Thomas A. ; Zatsepin, Nadia ; Shilova, Anastasya ; Nelson, Garrett ; Liu, Haiguang ; Johansson, Linda ; Heymann, Michael ; Jaeger, Kathrin ; Metz, Markus ; Wickstrand, Cecilia ; Wu, Wenting ; Båth, Petra ; Berntsen, Peter ; Oberthuer, Dominik ; Panneels, Valerie ; Cherezov, Vadim ; Chapman, Henry ; Schertler, Gebhard ; Neutze, Richard ; Spence, John ; Moraes, Isabel ; Burghammer, Manfred ; Standfuss, Joerg ; Weierstall, Uwe. / Lipidic cubic phase serial millisecond crystallography using synchrotron radiation. In: IUCrJ. 2015 ; Vol. 2. pp. 168-176.
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