Lipidic cubic phase serial millisecond crystallography using synchrotron radiation

Przemyslaw Nogly, Daniel James, Dingjie Wang, Thomas A. White, Nadia Zatsepin, Anastasya Shilova, Garrett Nelson, Haiguang Liu, Linda Johansson, Michael Heymann, Kathrin Jaeger, Markus Metz, Cecilia Wickstrand, Wenting Wu, Petra Båth, Peter Berntsen, Dominik Oberthuer, Valerie Panneels, Vadim Cherezov, Henry Chapman & 7 others Gebhard Schertler, Richard Neutze, John Spence, Isabel Moraes, Manfred Burghammer, Joerg Standfuss, Uwe Weierstall

    Research output: Contribution to journalArticle

    87 Citations (Scopus)

    Abstract

    Lipidic cubic phases (LCPs) have emerged as successful matrixes for the crystallization of membrane proteins. Moreover, the viscous LCP also provides a highly effective delivery medium for serial femtosecond crystallography (SFX) at X-ray free-electron lasers (XFELs). Here, the adaptation of this technology to perform serial millisecond crystallography (SMX) at more widely available synchrotron microfocus beamlines is described. Compared with conventional microcrystallography, LCP-SMX eliminates the need for difficult handling of individual crystals and allows for data collection at room temperature. The technology is demonstrated by solving a structure of the light-driven proton-pump bacteriorhodopsin (bR) at a resolution of 2.4±Å. The room-temperature structure of bR is very similar to previous cryogenic structures but shows small yet distinct differences in the retinal ligand and proton-transfer pathway.

    Original languageEnglish (US)
    Pages (from-to)168-176
    Number of pages9
    JournalIUCrJ
    Volume2
    DOIs
    StatePublished - Feb 26 2015

    Fingerprint

    Bacteriorhodopsins
    Crystallography
    Synchrotrons
    Synchrotron radiation
    crystallography
    synchrotron radiation
    Radiation
    X ray lasers
    Technology
    Proton Pumps
    Temperature
    Proton transfer
    protons
    Free electron lasers
    room temperature
    Crystallization
    free electron lasers
    Cryogenics
    cryogenics
    Protons

    Keywords

    • bacteriorhodopsin
    • lipidic cubic phases
    • protein crystallography
    • XFEL

    ASJC Scopus subject areas

    • Chemistry(all)
    • Materials Science(all)
    • Condensed Matter Physics
    • Biochemistry

    Cite this

    Lipidic cubic phase serial millisecond crystallography using synchrotron radiation. / Nogly, Przemyslaw; James, Daniel; Wang, Dingjie; White, Thomas A.; Zatsepin, Nadia; Shilova, Anastasya; Nelson, Garrett; Liu, Haiguang; Johansson, Linda; Heymann, Michael; Jaeger, Kathrin; Metz, Markus; Wickstrand, Cecilia; Wu, Wenting; Båth, Petra; Berntsen, Peter; Oberthuer, Dominik; Panneels, Valerie; Cherezov, Vadim; Chapman, Henry; Schertler, Gebhard; Neutze, Richard; Spence, John; Moraes, Isabel; Burghammer, Manfred; Standfuss, Joerg; Weierstall, Uwe.

    In: IUCrJ, Vol. 2, 26.02.2015, p. 168-176.

    Research output: Contribution to journalArticle

    Nogly, P, James, D, Wang, D, White, TA, Zatsepin, N, Shilova, A, Nelson, G, Liu, H, Johansson, L, Heymann, M, Jaeger, K, Metz, M, Wickstrand, C, Wu, W, Båth, P, Berntsen, P, Oberthuer, D, Panneels, V, Cherezov, V, Chapman, H, Schertler, G, Neutze, R, Spence, J, Moraes, I, Burghammer, M, Standfuss, J & Weierstall, U 2015, 'Lipidic cubic phase serial millisecond crystallography using synchrotron radiation' IUCrJ, vol. 2, pp. 168-176. https://doi.org/10.1107/S2052252514026487
    Nogly, Przemyslaw ; James, Daniel ; Wang, Dingjie ; White, Thomas A. ; Zatsepin, Nadia ; Shilova, Anastasya ; Nelson, Garrett ; Liu, Haiguang ; Johansson, Linda ; Heymann, Michael ; Jaeger, Kathrin ; Metz, Markus ; Wickstrand, Cecilia ; Wu, Wenting ; Båth, Petra ; Berntsen, Peter ; Oberthuer, Dominik ; Panneels, Valerie ; Cherezov, Vadim ; Chapman, Henry ; Schertler, Gebhard ; Neutze, Richard ; Spence, John ; Moraes, Isabel ; Burghammer, Manfred ; Standfuss, Joerg ; Weierstall, Uwe. / Lipidic cubic phase serial millisecond crystallography using synchrotron radiation. In: IUCrJ. 2015 ; Vol. 2. pp. 168-176.
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