Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography

Przemyslaw Nogly, Valerie Panneels, Garrett Nelson, Cornelius Gati, Tetsunari Kimura, Christopher Milne, Despina Milathianaki, Minoru Kubo, Wenting Wu, Chelsie Conrad, Jesse Coe, Richard Bean, Yun Zhao, Petra Båth, Robert Dods, Rajiv Harimoorthy, Kenneth R. Beyerlein, Jan Rheinberger, Daniel James, Daniel DePonte & 20 others Chufeng Li, Leonardo Sala, Garth J. Williams, Mark S. Hunter, Jason E. Koglin, Peter Berntsen, Eriko Nango, So Iwata, Henry N. Chapman, Petra Fromme, Matthias Frank, Rafael Abela, Sébastien Boutet, Anton Barty, Thomas A. White, Uwe Weierstall, John Spence, Richard Neutze, Gebhard Schertler, Jörg Standfuss

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Serial femtosecond crystallography (SFX) using X-ray free-electron laser sources is an emerging method with considerable potential for time-resolved pump-probe experiments. Here we present a lipidic cubic phase SFX structure of the light-driven proton pump bacteriorhodopsin (bR) to 2.3 Å resolution and a method to investigate protein dynamics with modest sample requirement. Time-resolved SFX (TR-SFX) with a pump-probe delay of 1 ms yields difference Fourier maps compatible with the dark to M state transition of bR. Importantly, the method is very sample efficient and reduces sample consumption to about 1 mg per collected time point. Accumulation of M intermediate within the crystal lattice is confirmed by time-resolved visible absorption spectroscopy. This study provides an important step towards characterizing the complete photocycle dynamics of retinal proteins and demonstrates the feasibility of a sample efficient viscous medium jet for TR-SFX.

Original languageEnglish (US)
Article number12314
JournalNature Communications
Volume7
DOIs
StatePublished - Aug 22 2016

Fingerprint

Bacteriorhodopsins
Crystallography
injectors
crystallography
delivery
Pumps
X ray lasers
Proton Pumps
Crystals
Free electron lasers
Phase structure
Absorption spectroscopy
Crystal lattices
crystals
Light sources
pumps
Proteins
proteins
probes
free electron lasers

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Nogly, P., Panneels, V., Nelson, G., Gati, C., Kimura, T., Milne, C., ... Standfuss, J. (2016). Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography. Nature Communications, 7, [12314]. https://doi.org/10.1038/ncomms12314

Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography. / Nogly, Przemyslaw; Panneels, Valerie; Nelson, Garrett; Gati, Cornelius; Kimura, Tetsunari; Milne, Christopher; Milathianaki, Despina; Kubo, Minoru; Wu, Wenting; Conrad, Chelsie; Coe, Jesse; Bean, Richard; Zhao, Yun; Båth, Petra; Dods, Robert; Harimoorthy, Rajiv; Beyerlein, Kenneth R.; Rheinberger, Jan; James, Daniel; DePonte, Daniel; Li, Chufeng; Sala, Leonardo; Williams, Garth J.; Hunter, Mark S.; Koglin, Jason E.; Berntsen, Peter; Nango, Eriko; Iwata, So; Chapman, Henry N.; Fromme, Petra; Frank, Matthias; Abela, Rafael; Boutet, Sébastien; Barty, Anton; White, Thomas A.; Weierstall, Uwe; Spence, John; Neutze, Richard; Schertler, Gebhard; Standfuss, Jörg.

In: Nature Communications, Vol. 7, 12314, 22.08.2016.

Research output: Contribution to journalArticle

Nogly, P, Panneels, V, Nelson, G, Gati, C, Kimura, T, Milne, C, Milathianaki, D, Kubo, M, Wu, W, Conrad, C, Coe, J, Bean, R, Zhao, Y, Båth, P, Dods, R, Harimoorthy, R, Beyerlein, KR, Rheinberger, J, James, D, DePonte, D, Li, C, Sala, L, Williams, GJ, Hunter, MS, Koglin, JE, Berntsen, P, Nango, E, Iwata, S, Chapman, HN, Fromme, P, Frank, M, Abela, R, Boutet, S, Barty, A, White, TA, Weierstall, U, Spence, J, Neutze, R, Schertler, G & Standfuss, J 2016, 'Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography', Nature Communications, vol. 7, 12314. https://doi.org/10.1038/ncomms12314
Nogly, Przemyslaw ; Panneels, Valerie ; Nelson, Garrett ; Gati, Cornelius ; Kimura, Tetsunari ; Milne, Christopher ; Milathianaki, Despina ; Kubo, Minoru ; Wu, Wenting ; Conrad, Chelsie ; Coe, Jesse ; Bean, Richard ; Zhao, Yun ; Båth, Petra ; Dods, Robert ; Harimoorthy, Rajiv ; Beyerlein, Kenneth R. ; Rheinberger, Jan ; James, Daniel ; DePonte, Daniel ; Li, Chufeng ; Sala, Leonardo ; Williams, Garth J. ; Hunter, Mark S. ; Koglin, Jason E. ; Berntsen, Peter ; Nango, Eriko ; Iwata, So ; Chapman, Henry N. ; Fromme, Petra ; Frank, Matthias ; Abela, Rafael ; Boutet, Sébastien ; Barty, Anton ; White, Thomas A. ; Weierstall, Uwe ; Spence, John ; Neutze, Richard ; Schertler, Gebhard ; Standfuss, Jörg. / Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography. In: Nature Communications. 2016 ; Vol. 7.
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AU - Milne, Christopher

AU - Milathianaki, Despina

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AU - Dods, Robert

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AU - Beyerlein, Kenneth R.

AU - Rheinberger, Jan

AU - James, Daniel

AU - DePonte, Daniel

AU - Li, Chufeng

AU - Sala, Leonardo

AU - Williams, Garth J.

AU - Hunter, Mark S.

AU - Koglin, Jason E.

AU - Berntsen, Peter

AU - Nango, Eriko

AU - Iwata, So

AU - Chapman, Henry N.

AU - Fromme, Petra

AU - Frank, Matthias

AU - Abela, Rafael

AU - Boutet, Sébastien

AU - Barty, Anton

AU - White, Thomas A.

AU - Weierstall, Uwe

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