Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography

Przemyslaw Nogly; Valerie Panneels; Garrett Nelson; Cornelius Gati; Tetsunari Kimura; Christopher Milne; Despina Milathianaki; Minoru Kubo; Wenting Wu; Chelsie Conrad; Jesse Coe; Richard Bean; Yun Zhao; Petra Båth; Robert Dods; Rajiv Harimoorthy; Kenneth R. Beyerlein; Jan Rheinberger; Daniel James; Daniel DePonte; Chufeng Li; Leonardo Sala; Garth J. Williams; Mark S. Hunter; Jason E. Koglin; Peter Berntsen; Eriko Nango; So Iwata; Henry N. Chapman; Petra Fromme; Matthias Frank; Rafael Abela; Sébastien Boutet; Anton Barty; Thomas A. White; Uwe Weierstall; John Spence; Richard Neutze; Gebhard Schertler; Jörg Standfuss

doi:10.1038/ncomms12314

Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography

Przemyslaw Nogly, Valerie Panneels, Garrett Nelson, Cornelius Gati, Tetsunari Kimura, Christopher Milne, Despina Milathianaki, Minoru Kubo, Wenting Wu, Chelsie Conrad, Jesse Coe, Richard Bean, Yun Zhao, Petra Båth, Robert Dods, Rajiv Harimoorthy, Kenneth R. Beyerlein, Jan Rheinberger, Daniel James, Daniel DePonteChufeng Li, Leonardo Sala, Garth J. Williams, Mark S. Hunter, Jason E. Koglin, Peter Berntsen, Eriko Nango, So Iwata, Henry N. Chapman, Petra Fromme, Matthias Frank, Rafael Abela, Sébastien Boutet, Anton Barty, Thomas A. White, Uwe Weierstall, John Spence, Richard Neutze, Gebhard Schertler, Jörg Standfuss

Research output: Contribution to journal › Article › peer-review

69 Scopus citations

Abstract

Serial femtosecond crystallography (SFX) using X-ray free-electron laser sources is an emerging method with considerable potential for time-resolved pump-probe experiments. Here we present a lipidic cubic phase SFX structure of the light-driven proton pump bacteriorhodopsin (bR) to 2.3 Å resolution and a method to investigate protein dynamics with modest sample requirement. Time-resolved SFX (TR-SFX) with a pump-probe delay of 1 ms yields difference Fourier maps compatible with the dark to M state transition of bR. Importantly, the method is very sample efficient and reduces sample consumption to about 1 mg per collected time point. Accumulation of M intermediate within the crystal lattice is confirmed by time-resolved visible absorption spectroscopy. This study provides an important step towards characterizing the complete photocycle dynamics of retinal proteins and demonstrates the feasibility of a sample efficient viscous medium jet for TR-SFX.

Original language	English (US)
Article number	12314
Journal	Nature communications
Volume	7
DOIs	https://doi.org/10.1038/ncomms12314
State	Published - Aug 22 2016

ASJC Scopus subject areas

General Chemistry
General Biochemistry, Genetics and Molecular Biology
General
General Physics and Astronomy

Access to Document

10.1038/ncomms12314

Cite this

Nogly, P., Panneels, V., Nelson, G., Gati, C., Kimura, T., Milne, C., Milathianaki, D., Kubo, M., Wu, W., Conrad, C., Coe, J., Bean, R., Zhao, Y., Båth, P., Dods, R., Harimoorthy, R., Beyerlein, K. R., Rheinberger, J., James, D., ... Standfuss, J. (2016). Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography. Nature communications, 7, Article 12314. https://doi.org/10.1038/ncomms12314

Nogly, P, Panneels, V, Nelson, G, Gati, C, Kimura, T, Milne, C, Milathianaki, D, Kubo, M, Wu, W, Conrad, C, Coe, J, Bean, R, Zhao, Y, Båth, P, Dods, R, Harimoorthy, R, Beyerlein, KR, Rheinberger, J, James, D, DePonte, D, Li, C, Sala, L, Williams, GJ, Hunter, MS, Koglin, JE, Berntsen, P, Nango, E, Iwata, S, Chapman, HN, Fromme, P, Frank, M, Abela, R, Boutet, S, Barty, A, White, TA, Weierstall, U, Spence, J, Neutze, R, Schertler, G & Standfuss, J 2016, 'Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography', Nature communications, vol. 7, 12314. https://doi.org/10.1038/ncomms12314

@article{0f3962edb30444e7b3c9714c364c5404,

title = "Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography",

abstract = "Serial femtosecond crystallography (SFX) using X-ray free-electron laser sources is an emerging method with considerable potential for time-resolved pump-probe experiments. Here we present a lipidic cubic phase SFX structure of the light-driven proton pump bacteriorhodopsin (bR) to 2.3 {\AA} resolution and a method to investigate protein dynamics with modest sample requirement. Time-resolved SFX (TR-SFX) with a pump-probe delay of 1 ms yields difference Fourier maps compatible with the dark to M state transition of bR. Importantly, the method is very sample efficient and reduces sample consumption to about 1 mg per collected time point. Accumulation of M intermediate within the crystal lattice is confirmed by time-resolved visible absorption spectroscopy. This study provides an important step towards characterizing the complete photocycle dynamics of retinal proteins and demonstrates the feasibility of a sample efficient viscous medium jet for TR-SFX.",

author = "Przemyslaw Nogly and Valerie Panneels and Garrett Nelson and Cornelius Gati and Tetsunari Kimura and Christopher Milne and Despina Milathianaki and Minoru Kubo and Wenting Wu and Chelsie Conrad and Jesse Coe and Richard Bean and Yun Zhao and Petra B{\aa}th and Robert Dods and Rajiv Harimoorthy and Beyerlein, {Kenneth R.} and Jan Rheinberger and Daniel James and Daniel DePonte and Chufeng Li and Leonardo Sala and Williams, {Garth J.} and Hunter, {Mark S.} and Koglin, {Jason E.} and Peter Berntsen and Eriko Nango and So Iwata and Chapman, {Henry N.} and Petra Fromme and Matthias Frank and Rafael Abela and S{\'e}bastien Boutet and Anton Barty and White, {Thomas A.} and Uwe Weierstall and John Spence and Richard Neutze and Gebhard Schertler and J{\"o}rg Standfuss",

note = "Funding Information: This work was further supported by the X-ray Free-Electron Laser Priority Strategy Program and by the Japan Science and Technology agency. Parts of the sample injector used at LCLS for this research was funded by the National Institutes of Health, P41GM103393, formerly P41RR001209",

year = "2016",

month = aug,

day = "22",

doi = "10.1038/ncomms12314",

language = "English (US)",

volume = "7",

journal = "Nature communications",

issn = "2041-1723",

publisher = "Nature Publishing Group",

}

TY - JOUR

T1 - Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography

AU - Nogly, Przemyslaw

AU - Panneels, Valerie

AU - Nelson, Garrett

AU - Gati, Cornelius

AU - Kimura, Tetsunari

AU - Milne, Christopher

AU - Milathianaki, Despina

AU - Kubo, Minoru

AU - Wu, Wenting

AU - Conrad, Chelsie

AU - Coe, Jesse

AU - Bean, Richard

AU - Zhao, Yun

AU - Båth, Petra

AU - Dods, Robert

AU - Harimoorthy, Rajiv

AU - Beyerlein, Kenneth R.

AU - Rheinberger, Jan

AU - James, Daniel

AU - DePonte, Daniel

AU - Li, Chufeng

AU - Sala, Leonardo

AU - Williams, Garth J.

AU - Hunter, Mark S.

AU - Koglin, Jason E.

AU - Berntsen, Peter

AU - Nango, Eriko

AU - Iwata, So

AU - Chapman, Henry N.

AU - Fromme, Petra

AU - Frank, Matthias

AU - Abela, Rafael

AU - Boutet, Sébastien

AU - Barty, Anton

AU - White, Thomas A.

AU - Weierstall, Uwe

AU - Spence, John

AU - Neutze, Richard

AU - Schertler, Gebhard

AU - Standfuss, Jörg

N1 - Funding Information: This work was further supported by the X-ray Free-Electron Laser Priority Strategy Program and by the Japan Science and Technology agency. Parts of the sample injector used at LCLS for this research was funded by the National Institutes of Health, P41GM103393, formerly P41RR001209

PY - 2016/8/22

Y1 - 2016/8/22

N2 - Serial femtosecond crystallography (SFX) using X-ray free-electron laser sources is an emerging method with considerable potential for time-resolved pump-probe experiments. Here we present a lipidic cubic phase SFX structure of the light-driven proton pump bacteriorhodopsin (bR) to 2.3 Å resolution and a method to investigate protein dynamics with modest sample requirement. Time-resolved SFX (TR-SFX) with a pump-probe delay of 1 ms yields difference Fourier maps compatible with the dark to M state transition of bR. Importantly, the method is very sample efficient and reduces sample consumption to about 1 mg per collected time point. Accumulation of M intermediate within the crystal lattice is confirmed by time-resolved visible absorption spectroscopy. This study provides an important step towards characterizing the complete photocycle dynamics of retinal proteins and demonstrates the feasibility of a sample efficient viscous medium jet for TR-SFX.

AB - Serial femtosecond crystallography (SFX) using X-ray free-electron laser sources is an emerging method with considerable potential for time-resolved pump-probe experiments. Here we present a lipidic cubic phase SFX structure of the light-driven proton pump bacteriorhodopsin (bR) to 2.3 Å resolution and a method to investigate protein dynamics with modest sample requirement. Time-resolved SFX (TR-SFX) with a pump-probe delay of 1 ms yields difference Fourier maps compatible with the dark to M state transition of bR. Importantly, the method is very sample efficient and reduces sample consumption to about 1 mg per collected time point. Accumulation of M intermediate within the crystal lattice is confirmed by time-resolved visible absorption spectroscopy. This study provides an important step towards characterizing the complete photocycle dynamics of retinal proteins and demonstrates the feasibility of a sample efficient viscous medium jet for TR-SFX.

UR - http://www.scopus.com/inward/record.url?scp=84983749136&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84983749136&partnerID=8YFLogxK

U2 - 10.1038/ncomms12314

DO - 10.1038/ncomms12314

M3 - Article

C2 - 27545823

AN - SCOPUS:84983749136

SN - 2041-1723

VL - 7

JO - Nature communications

JF - Nature communications

M1 - 12314

ER -

Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this