Light stimulation of conidiation in Neurospora crassa: Studies with the wild-type strain and mutants wc-1, wc-2 and acon-2

Frank Roman Lauter, Carl T. Yamashiro, Charles Yanofsky

Research output: Contribution to journalArticlepeer-review

37 Scopus citations

Abstract

Blue light influences several events in the life cycle of the ascomycete Neurospora crassa. It has been shown previously that light stimulates asexual sporulation (conidiation) in the double mutant strain, albino-2, band (al-2 bd). In this paper, we report that light is also essential for maximal conidiation by the wild-type strain. Consistent with this finding, two mutants which are defective in other light responses, wc-1 and wc-2, conidiate as efficiently as the wild-type strain in the dark, but show no incremental response to light. Light has a profound effect on conidiation in acon-2, a mutant which is temperature sensitive for conidiation. At the permissible temperature, this mutant produces approximately 500-fold more conidia in the light than in the dark. In addition, the double mutants acon-2 wc-1 and acon-2 wc-2 conidiate very poorly, even in the light. Microscopic studies have shown that the majority of aerial hyphae produced by the acon-2 wc-1 and acon-2 wc-2 double mutants at the non-permissible temperature are blocked at the same stage of development as observed for their single mutant parent acon-2, namely at the stage leading to minor constriction chain formation. Northern blot analyses examining the expression of two conidiation-specific genes are consistent with this conclusion.

Original languageEnglish (US)
Pages (from-to)203-211
Number of pages9
JournalJournal of Photochemistry and Photobiology B: Biology
Volume37
Issue number3
DOIs
StatePublished - Feb 1997

Keywords

  • Aconidial mutant
  • Conidiation
  • Light effects

ASJC Scopus subject areas

  • Radiation
  • Radiological and Ultrasound Technology
  • Biophysics
  • Radiology Nuclear Medicine and imaging

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