Kinetics of small molecule interactions with membrane proteins in single cells measured with mechanical amplification

Yan Guan, Xiaonan Shan, Fenni Zhang, Shaopeng Wang, Hong Yuan Chen, Nongjian Tao

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Measuring small molecule interactions with membrane proteins in single cells is critical for understanding many cellular processes and for screening drugs. However, developing such a capability has been a difficult challenge. We show that molecular interactions with membrane proteins induce a mechanical deformation in the cellular membrane, and real-time monitoring of the deformation with subnanometer resolution allows quantitative analysis of small molecule-membrane protein interaction kinetics in single cells. This new strategy provides mechanical amplification of small binding signals, making it possible to detect small molecule interactions with membrane proteins. This capability, together with spatial resolution, also allows the study of the heterogeneous nature of cells by analyzing the interaction kinetics variability between different cells and between different regions of a single cell.

Original languageEnglish (US)
Article number1500633
JournalScience Advances
Volume1
Issue number9
DOIs
StatePublished - Oct 2015

ASJC Scopus subject areas

  • General

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