Isolation of two genes that affect mitotic chromosome transmission in S. cerevisiae

Douglas Meeks-Wagner, John S. Wood, Barbara Garvik, Leland Hartwell

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Two DNA sequences that reduce mitotic fidelity of chromosome transmission have been identified: MIF1 and MIF2. MIF1 is a unique sequence located on the right arm of chromosome XII that stimulates loss and recombination for both chromosomes V and VII when present in a high copy number plasmid. MIF1 is not essential for cell division but is necessary for the normal fidelity of chromosome transmission. MIF2 is a unique sequence located 15 cM distal to HIS6 on chromosome IX that induces a high frequency of chromosome VII loss and a lower frequency of chromosome V loss when present in high copy number; it has no effect on mitotic recombination. Disruption of the genomic MIF2 locus was lethal and cells lacking this function arrested division with a terminal phenotype characteristic of a block in DNA replication or nuclear division.

Original languageEnglish (US)
Pages (from-to)53-63
Number of pages11
JournalCell
Volume44
Issue number1
DOIs
StatePublished - Jan 17 1986
Externally publishedYes

Fingerprint

Chromosomes
Saccharomyces cerevisiae
Genes
Genetic Recombination
Cell Nucleus Division
DNA sequences
DNA Replication
Cell Division
Plasmids
Cells
Phenotype
DNA

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this

Isolation of two genes that affect mitotic chromosome transmission in S. cerevisiae. / Meeks-Wagner, Douglas; Wood, John S.; Garvik, Barbara; Hartwell, Leland.

In: Cell, Vol. 44, No. 1, 17.01.1986, p. 53-63.

Research output: Contribution to journalArticle

Meeks-Wagner, Douglas ; Wood, John S. ; Garvik, Barbara ; Hartwell, Leland. / Isolation of two genes that affect mitotic chromosome transmission in S. cerevisiae. In: Cell. 1986 ; Vol. 44, No. 1. pp. 53-63.
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