Isolation of the replication and partitioning regions of the Salmonella typhimurium virulence plasmid and stabilization of heterologous replicons

S. A. Tinge, R. Curtiss

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Although the virulence plasmid of Salmonella typhimurium has a copy number of one to two per chromosome, plasmid-free segregants are produced at a rate less than 10-7 per cell per generation. Three regions appear to be involved in the maintenance of this virulence plasmid. The first two, repB and repC, are functional replicons hybridizing with IncFII and IncFI plasmids, respectively, neither exhibiting the segregational stability of the parent virulence plasmid. The third region, par, cloned as a 3.9-kilobase Sau3A fragment, is not a functional replicon but exhibits incompatibility with the virulence plasmid. Subsequent tests revealed the ability of this 3.9-kilobase par insert to increase the stability of pACYC184 in S. typhimurium from less than 34% to 99% plasmid-containing cells after 50 generations. In addition, the par region increased the stability of oriC, R388, and repC replicons in both S. typhimurium and Escherichia coli hosts. The par region encodes 44,000- and 40,000-molecular-weight proteins essential for the Par+ phenotype but not for the Inc+ phenotype. Although actual sequestering of plasmids within the cell was not demonstrated, all results indicate that the par region described is an actual partitioning locus, similar in organization to those described for plasmids F, P1, and NR1.

Original languageEnglish (US)
Pages (from-to)5266-5277
Number of pages12
JournalJournal of Bacteriology
Volume172
Issue number9
StatePublished - 1990
Externally publishedYes

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Replicon
Salmonella typhimurium
Virulence
Plasmids
R388
F Factor
Phenotype
Chromosomes
Molecular Weight
Maintenance
Escherichia coli

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Isolation of the replication and partitioning regions of the Salmonella typhimurium virulence plasmid and stabilization of heterologous replicons. / Tinge, S. A.; Curtiss, R.

In: Journal of Bacteriology, Vol. 172, No. 9, 1990, p. 5266-5277.

Research output: Contribution to journalArticle

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