Isolation, characterization and nucleotide sequence of the Streptococcus mutans lactose-specific Enzyme II (lacE) gene of the PTS and the phospho-β-galactosidase (lacG) gene

A. L. Honeyman, R. Curtiss

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

The lacE and lacG genes from Streptococcus mutans have been isolated and characterized, and their nucleotide sequence has been determined. The lacE gene encodes the lactose-specific Enzyme II component of the phosphoenolpyruvate-dependent phosphotransferase system (PTS). The lacG gene encodes the phospho-β-galactosidase which cleaves the lactose phosphate that is formed by the lactose PTS. The S. mutans lacE and lacG genes are located in the same operon as the tagatose genes. S. mutans metabolizes lactose via the tagatose phosphate pathway. The deduced LacE and LacG proteins of S. mutans display high homology with the mg proteins from Lactococcus lactis, Staphylococcus aureus and Lactobacillus casei.

Original languageEnglish (US)
Pages (from-to)2685-2694
Number of pages10
JournalJournal of General Microbiology
Volume139
Issue number11
DOIs
StatePublished - 1993

ASJC Scopus subject areas

  • Microbiology

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