Isolation and characterization of conjugation deficient mutants of Escherichia coli K 12

J. O. Falkinham, R. Curtiss

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Conjugation deficient mutants (Con-) of Escherichia coli K 12 have been isolated by a variety of indirect selective techniques. Mutants with mutations conferring ampicillin resistance, fosfomycin resistance, an alanine requirement, and a failure to ferment a number of carbohydrates were selected because the impaired functions occur in association with cell wall and cell membrane defects. The integrity of these catalytic or structural elements is postulated to have a role in conjugation. The mutants could be divided into at least six general categories corresponding to their defectiveness in the following postulated recipient cell functions: (i) specific union formation, (ii) effective union formation, (iii) deoxyribonucleic acid transfer, (iv) plasmid establishment, (v) plasmid maintenance, and (vi) recombination. The availability of these mutants should contribute to the description of the molecular events involved in each of these conjugation steps and the elucidation of the genetic control over the inheritance of conjugationally transferred deoxyribonucleic acid.

Original languageEnglish (US)
Pages (from-to)1194-1206
Number of pages13
JournalJournal of bacteriology
Volume126
Issue number3
StatePublished - Dec 1 1976

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Fingerprint Dive into the research topics of 'Isolation and characterization of conjugation deficient mutants of Escherichia coli K 12'. Together they form a unique fingerprint.

Cite this