IRS and tor nutrient-signaling pathways act via juvenile hormone to influence honey bee caste fate

Navdeep S. Mutti, Adam G. Dolezal, Florian Wolschin, Jasdeep S. Mutti, Kulvinder S. Gill, Gro Amdam

Research output: Contribution to journalArticle

116 Scopus citations

Abstract

Regardless of genetic makeup, a female honey bee becomes a queen or worker depending on the food she receives as a larva. For decades, it has been known that nutrition and juvenile hormone (JH) signaling determine the caste fate of the individual bee. However, it is still largely unclear how these factors are connected. To address this question, we suppressed nutrient sensing by RNA interference (RNAi)-mediated gene knockdown of IRS (insulin receptor substrate) and TOR (target of rapamycin) in larvae reared on queen diet. The treatments affected several layers of organismal organization that could play a role in the response to differential nutrition between castes. These include transcript profiles, proteomic patterns, lipid levels, DNA methylation response and morphological features. Most importantly, gene knockdown abolished a JH peak that signals queen development and resulted in a worker phenotype. Application of JH rescued the queen phenotype in either knockdown, which demonstrates that the larval response to JH remains intact and can drive normal developmental plasticity even when IRS or TOR transcript levels are reduced. We discuss our results in the context of other recent findings on honey bee caste and development and propose that IRS is an alternative substrate for the Egfr (epidermal growth factor receptor) in honey bees. Overall, our study describes how the interplay of nutritional and hormonal signals affects many levels of organismal organization to build different phenotypes from identical genotypes.

Original languageEnglish (US)
Pages (from-to)3977-3984
Number of pages8
JournalJournal of Experimental Biology
Volume214
Issue number23
DOIs
StatePublished - Dec 2011

Keywords

  • Diphenic development
  • Epidermal growth factor receptor
  • Insulin/insulin-like signaling
  • Queen bee
  • Target of rapamycin
  • Worker bee

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Physiology
  • Aquatic Science
  • Animal Science and Zoology
  • Molecular Biology
  • Insect Science

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