Iron deficiency decreases gluconeogenesis in isolated rat hepatocytes

K. L. Klempa, W. T. Willis, R. Chengson, P. R. Dallman, G. A. Brooks

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Dietary iron deficiency in rats results in increased blood glucose turnover and recycling. We measured the rates of glucose production in isolated hepatocytes from iron-sufficient (Fe+) and iron-deficient (Fe-) rats to assess the intrinsic capacity of the Fe- liver to carry out gluconeogenesis. Low-iron and control diets were given to 21-day-old female rats. After 4-5 wk, hemoglobin concentrations averaged 4.1 g/dl in the Fe- and 14.3 g/dl in the Fe+ animals. In the hepatocytes from Fe- rats, there was a 35% decrease in the rate of glucose production from 1 mM pyruvate + 10 mM lactate, a 48% decrease from 0.1 mM pyruvate + 1 mM lactate, a 39% decrease from 1 mM alanine, and a 48% decrease from 1 mM glycerol. The addition of 5 μM norepinephrine or 0.5 μM glucagon to the incubation media produced stimulatory effects on hepatocytes from both Fe- and Fe+ rats, resulting in the maintenance of an average difference of 38% in the rates of gluconeogenesis between the two groups. Studies on isolated liver mitochondria and cytosol revealed α-glycerophosphate-cytochrome c reductase and phospho(enol)pyruvate carboxykinase activities to be decreased by 27% in Fe- rats. We conclude that because severe dietary iron deficiency decreases gluconeogenesis in isolated rat hepatocytes, the increased gluconeogenesis demonstrated by Fe- rats in vivo is attributable to increased availability of gluconeogenic substrates and upregulation of the pathway.

Original languageEnglish (US)
Pages (from-to)1868-1872
Number of pages5
JournalJournal of Applied Physiology
Volume67
Issue number5
StatePublished - 1989
Externally publishedYes

Fingerprint

Gluconeogenesis
Hepatocytes
Iron
Pyruvic Acid
Dietary Iron
Lactic Acid
Cytochrome Reductases
Glycerophosphates
Glucose
Liver Mitochondrion
Recycling
Cytochromes c
Glucagon
Alanine
Cytosol
Glycerol
Blood Glucose
Norepinephrine
Hemoglobins
Up-Regulation

Keywords

  • Exertion
  • glucose
  • mitochondria

ASJC Scopus subject areas

  • Endocrinology
  • Physiology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Klempa, K. L., Willis, W. T., Chengson, R., Dallman, P. R., & Brooks, G. A. (1989). Iron deficiency decreases gluconeogenesis in isolated rat hepatocytes. Journal of Applied Physiology, 67(5), 1868-1872.

Iron deficiency decreases gluconeogenesis in isolated rat hepatocytes. / Klempa, K. L.; Willis, W. T.; Chengson, R.; Dallman, P. R.; Brooks, G. A.

In: Journal of Applied Physiology, Vol. 67, No. 5, 1989, p. 1868-1872.

Research output: Contribution to journalArticle

Klempa, KL, Willis, WT, Chengson, R, Dallman, PR & Brooks, GA 1989, 'Iron deficiency decreases gluconeogenesis in isolated rat hepatocytes', Journal of Applied Physiology, vol. 67, no. 5, pp. 1868-1872.
Klempa KL, Willis WT, Chengson R, Dallman PR, Brooks GA. Iron deficiency decreases gluconeogenesis in isolated rat hepatocytes. Journal of Applied Physiology. 1989;67(5):1868-1872.
Klempa, K. L. ; Willis, W. T. ; Chengson, R. ; Dallman, P. R. ; Brooks, G. A. / Iron deficiency decreases gluconeogenesis in isolated rat hepatocytes. In: Journal of Applied Physiology. 1989 ; Vol. 67, No. 5. pp. 1868-1872.
@article{a1282baf5faa4200b28b8ad5aec91b05,
title = "Iron deficiency decreases gluconeogenesis in isolated rat hepatocytes",
abstract = "Dietary iron deficiency in rats results in increased blood glucose turnover and recycling. We measured the rates of glucose production in isolated hepatocytes from iron-sufficient (Fe+) and iron-deficient (Fe-) rats to assess the intrinsic capacity of the Fe- liver to carry out gluconeogenesis. Low-iron and control diets were given to 21-day-old female rats. After 4-5 wk, hemoglobin concentrations averaged 4.1 g/dl in the Fe- and 14.3 g/dl in the Fe+ animals. In the hepatocytes from Fe- rats, there was a 35{\%} decrease in the rate of glucose production from 1 mM pyruvate + 10 mM lactate, a 48{\%} decrease from 0.1 mM pyruvate + 1 mM lactate, a 39{\%} decrease from 1 mM alanine, and a 48{\%} decrease from 1 mM glycerol. The addition of 5 μM norepinephrine or 0.5 μM glucagon to the incubation media produced stimulatory effects on hepatocytes from both Fe- and Fe+ rats, resulting in the maintenance of an average difference of 38{\%} in the rates of gluconeogenesis between the two groups. Studies on isolated liver mitochondria and cytosol revealed α-glycerophosphate-cytochrome c reductase and phospho(enol)pyruvate carboxykinase activities to be decreased by 27{\%} in Fe- rats. We conclude that because severe dietary iron deficiency decreases gluconeogenesis in isolated rat hepatocytes, the increased gluconeogenesis demonstrated by Fe- rats in vivo is attributable to increased availability of gluconeogenic substrates and upregulation of the pathway.",
keywords = "Exertion, glucose, mitochondria",
author = "Klempa, {K. L.} and Willis, {W. T.} and R. Chengson and Dallman, {P. R.} and Brooks, {G. A.}",
year = "1989",
language = "English (US)",
volume = "67",
pages = "1868--1872",
journal = "Journal of Applied Physiology",
issn = "8750-7587",
publisher = "American Physiological Society",
number = "5",

}

TY - JOUR

T1 - Iron deficiency decreases gluconeogenesis in isolated rat hepatocytes

AU - Klempa, K. L.

AU - Willis, W. T.

AU - Chengson, R.

AU - Dallman, P. R.

AU - Brooks, G. A.

PY - 1989

Y1 - 1989

N2 - Dietary iron deficiency in rats results in increased blood glucose turnover and recycling. We measured the rates of glucose production in isolated hepatocytes from iron-sufficient (Fe+) and iron-deficient (Fe-) rats to assess the intrinsic capacity of the Fe- liver to carry out gluconeogenesis. Low-iron and control diets were given to 21-day-old female rats. After 4-5 wk, hemoglobin concentrations averaged 4.1 g/dl in the Fe- and 14.3 g/dl in the Fe+ animals. In the hepatocytes from Fe- rats, there was a 35% decrease in the rate of glucose production from 1 mM pyruvate + 10 mM lactate, a 48% decrease from 0.1 mM pyruvate + 1 mM lactate, a 39% decrease from 1 mM alanine, and a 48% decrease from 1 mM glycerol. The addition of 5 μM norepinephrine or 0.5 μM glucagon to the incubation media produced stimulatory effects on hepatocytes from both Fe- and Fe+ rats, resulting in the maintenance of an average difference of 38% in the rates of gluconeogenesis between the two groups. Studies on isolated liver mitochondria and cytosol revealed α-glycerophosphate-cytochrome c reductase and phospho(enol)pyruvate carboxykinase activities to be decreased by 27% in Fe- rats. We conclude that because severe dietary iron deficiency decreases gluconeogenesis in isolated rat hepatocytes, the increased gluconeogenesis demonstrated by Fe- rats in vivo is attributable to increased availability of gluconeogenic substrates and upregulation of the pathway.

AB - Dietary iron deficiency in rats results in increased blood glucose turnover and recycling. We measured the rates of glucose production in isolated hepatocytes from iron-sufficient (Fe+) and iron-deficient (Fe-) rats to assess the intrinsic capacity of the Fe- liver to carry out gluconeogenesis. Low-iron and control diets were given to 21-day-old female rats. After 4-5 wk, hemoglobin concentrations averaged 4.1 g/dl in the Fe- and 14.3 g/dl in the Fe+ animals. In the hepatocytes from Fe- rats, there was a 35% decrease in the rate of glucose production from 1 mM pyruvate + 10 mM lactate, a 48% decrease from 0.1 mM pyruvate + 1 mM lactate, a 39% decrease from 1 mM alanine, and a 48% decrease from 1 mM glycerol. The addition of 5 μM norepinephrine or 0.5 μM glucagon to the incubation media produced stimulatory effects on hepatocytes from both Fe- and Fe+ rats, resulting in the maintenance of an average difference of 38% in the rates of gluconeogenesis between the two groups. Studies on isolated liver mitochondria and cytosol revealed α-glycerophosphate-cytochrome c reductase and phospho(enol)pyruvate carboxykinase activities to be decreased by 27% in Fe- rats. We conclude that because severe dietary iron deficiency decreases gluconeogenesis in isolated rat hepatocytes, the increased gluconeogenesis demonstrated by Fe- rats in vivo is attributable to increased availability of gluconeogenic substrates and upregulation of the pathway.

KW - Exertion

KW - glucose

KW - mitochondria

UR - http://www.scopus.com/inward/record.url?scp=0024801845&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024801845&partnerID=8YFLogxK

M3 - Article

C2 - 2600020

AN - SCOPUS:0024801845

VL - 67

SP - 1868

EP - 1872

JO - Journal of Applied Physiology

JF - Journal of Applied Physiology

SN - 8750-7587

IS - 5

ER -