Insulator-based dielectrophoretic behavior of β- Galactosidase under DC and low frequency AC conditions

A. Nakano, F. Camacho-Alanis, Alexandra Ros

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

Dielectrophoresis (DEP) has been studied with a handful of proteins with insulator-based devices in recent years. Here, we extend these studies to β-galactosidase in comparison to previously studied DEP response of immunoglobulin G (IgG) under DC and AC conditions in insulator-based devices including nanoconstrictions. Our work indicates that β-galactosidase shows negative DEP under DC conditions. With an applied AC potential a decrease of concentration around the corresponding regions for negative DEP for β-galactosidase was observed with increasing applied frequencies. We assume this concentration decrease occurs due to an interplay of DEP, electrokinesis, diffusion, and potentially relaxation processes.

Original languageEnglish (US)
Title of host publication18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
PublisherChemical and Biological Microsystems Society
Pages2468-2470
Number of pages3
ISBN (Print)9780979806476
Publication statusPublished - 2014
Event18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States
Duration: Oct 26 2014Oct 30 2014

Other

Other18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
CountryUnited States
CitySan Antonio
Period10/26/1410/30/14

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Keywords

  • Dielectrophoresis
  • Insulator-based
  • Proteins
  • β-galactosidase

ASJC Scopus subject areas

  • Control and Systems Engineering

Cite this

Nakano, A., Camacho-Alanis, F., & Ros, A. (2014). Insulator-based dielectrophoretic behavior of β- Galactosidase under DC and low frequency AC conditions. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 (pp. 2468-2470). Chemical and Biological Microsystems Society.