Injector for scattering measurements on fully solvated biospecies

Research output: Contribution to journalArticle

144 Citations (Scopus)

Abstract

We describe a liquid jet injector system developed to deliver fully solvated microscopic target species into a probe beam under either vacuum or ambient conditions. The injector was designed specifically for x-ray scattering studies of biological nanospecies using x-ray free electron lasers and third generation synchrotrons, but is of interest to any application in which microscopic samples must be delivered in a fully solvated state and with microscopic precision. By utilizing a gas dynamic virtual nozzle (GDVN) to generate a sample-containing liquid jet of diameter ranging from 300 nm to 20 m, the injector avoids the clogging problems associated in this size range with conventional Rayleigh jets. A differential pumping system incorporated into the injector shields the experimental chamber from the gas load of the GDVN, making the injector compatible with high vacuum systems. The injector houses a fiber-optically coupled pump laser to illuminate the jet for pump-probe experiments and a hermetically sealed microscope to observe the liquid jet for diagnostics and alignment during operation. This injector system has now been used during several experimental runs at the Linac Coherent Light Source. Recent refinements in GDVN design are also presented.

Original languageEnglish (US)
Article number035108
JournalReview of Scientific Instruments
Volume83
Issue number3
DOIs
StatePublished - Mar 2012

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injectors
Gases
Scattering
Gas dynamics
Vacuum
scattering
gas dynamics
Lasers
X-Rays
Nozzles
Liquids
Synchrotrons
Pumps
Nozzle design
nozzles
X rays
Free electron lasers
liquids
nozzle design
pumps

ASJC Scopus subject areas

  • Instrumentation
  • Medicine(all)

Cite this

Injector for scattering measurements on fully solvated biospecies. / Weierstall, Uwe; Spence, John; Doak, R. B.

In: Review of Scientific Instruments, Vol. 83, No. 3, 035108, 03.2012.

Research output: Contribution to journalArticle

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