Inhibition of ICAM-mediated monocyte adhesion with a bioresponsive dextran-based conjugate

M. M. Kalstad, A. Panitch, G. R. Ehteshami, Stephen Massia

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The polysaccharide dextran, prepared as a colloid suspension in physiological saline, is routinely used as a plasma extender or substitute for emergency treatment of massive blood and fluid loss in settings where blood and blood products are not readily available. The objective of this study is to develop a dextran-peptide conjugate that selectively self-assembles onto injured tissue surfaces to form a protective colloid barrier against trauma-induced systemic inflammatory cell damage to healthy tissues. Endothelial cells were treated in vitro with a CD11b/CD18 antagonist conjugated to dextran. This agonist peptide conjugated to dextran decreased monocyte adhesion to TNF-α-activated endothelium more effectively than peptide alone. The scrambled version of the same peptide, conjugated and not, did not block monocyte adhesion in similarly activated endothelium. These studies indicate that an CD11b/CD18 antagonist dextran conjugate that is delivered introvascularly may limit organ damage following intestinal ischemia and reperfusion injury.

Original languageEnglish (US)
Title of host publicationAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Pages736-737
Number of pages2
Volume1
StatePublished - 2002
EventProceedings of the 2002 IEEE Engineering in Medicine and Biology 24th Annual Conference and the 2002 Fall Meeting of the Biomedical Engineering Society (BMES / EMBS) - Houston, TX, United States
Duration: Oct 23 2002Oct 26 2002

Other

OtherProceedings of the 2002 IEEE Engineering in Medicine and Biology 24th Annual Conference and the 2002 Fall Meeting of the Biomedical Engineering Society (BMES / EMBS)
CountryUnited States
CityHouston, TX
Period10/23/0210/26/02

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Keywords

  • Adhesion molecule
  • Dextran conjugate
  • ICAM
  • Immune response
  • Integrin
  • Plasma extender

ASJC Scopus subject areas

  • Bioengineering

Cite this

Kalstad, M. M., Panitch, A., Ehteshami, G. R., & Massia, S. (2002). Inhibition of ICAM-mediated monocyte adhesion with a bioresponsive dextran-based conjugate. In Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings (Vol. 1, pp. 736-737)