TY - JOUR
T1 - Inhibition of gene expression by RNase P
AU - Lundblad, Eirik Wasmuth
AU - Altman, Sidney
N1 - Funding Information:
We thank members of our laboratories for helpful discussions. This work was supported by a research grant from the Research Council of Norway (EWL) and Yale University.
PY - 2010/7/31
Y1 - 2010/7/31
N2 - The ability to interfere with gene expression is of crucial importance to unravel the function of genes and is also a promising therapeutic strategy. Here we discuss methodologies for inhibition of target RNAs based on the cleavage activity of the essential enzyme, Ribonuclease P (RNase P). RNase P-mediated cleavage of target RNAs can be directed by external guide sequences (EGSs) or by the use of the catalytic M1 RNA from E. coli linked to a guide sequence (M1GSs). These are not only basic tools for functional genetic studies in prokaryotic and eukaryotic cells but also promising antibacterial, anticancer and antiviral agents.
AB - The ability to interfere with gene expression is of crucial importance to unravel the function of genes and is also a promising therapeutic strategy. Here we discuss methodologies for inhibition of target RNAs based on the cleavage activity of the essential enzyme, Ribonuclease P (RNase P). RNase P-mediated cleavage of target RNAs can be directed by external guide sequences (EGSs) or by the use of the catalytic M1 RNA from E. coli linked to a guide sequence (M1GSs). These are not only basic tools for functional genetic studies in prokaryotic and eukaryotic cells but also promising antibacterial, anticancer and antiviral agents.
UR - http://www.scopus.com/inward/record.url?scp=77952881006&partnerID=8YFLogxK
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U2 - 10.1016/j.nbt.2010.03.003
DO - 10.1016/j.nbt.2010.03.003
M3 - Review article
C2 - 20211282
AN - SCOPUS:77952881006
SN - 1871-6784
VL - 27
SP - 212
EP - 221
JO - Gene Analysis Techniques
JF - Gene Analysis Techniques
IS - 3
ER -