Inhibition of gene expression by RNase P

Eirik Wasmuth Lundblad, Sidney Altman

Research output: Contribution to journalReview article

32 Scopus citations

Abstract

The ability to interfere with gene expression is of crucial importance to unravel the function of genes and is also a promising therapeutic strategy. Here we discuss methodologies for inhibition of target RNAs based on the cleavage activity of the essential enzyme, Ribonuclease P (RNase P). RNase P-mediated cleavage of target RNAs can be directed by external guide sequences (EGSs) or by the use of the catalytic M1 RNA from E. coli linked to a guide sequence (M1GSs). These are not only basic tools for functional genetic studies in prokaryotic and eukaryotic cells but also promising antibacterial, anticancer and antiviral agents.

Original languageEnglish (US)
Pages (from-to)212-221
Number of pages10
JournalNew Biotechnology
Volume27
Issue number3
DOIs
StatePublished - Jul 31 2010
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Molecular Biology

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