Inhibition of gene expression by RNase P

Eirik Wasmuth Lundblad; Sidney Altman

doi:10.1016/j.nbt.2010.03.003

Inhibition of gene expression by RNase P

Eirik Wasmuth Lundblad, Sidney Altman

Research output: Contribution to journal › Review article › peer-review

37 Scopus citations

Abstract

The ability to interfere with gene expression is of crucial importance to unravel the function of genes and is also a promising therapeutic strategy. Here we discuss methodologies for inhibition of target RNAs based on the cleavage activity of the essential enzyme, Ribonuclease P (RNase P). RNase P-mediated cleavage of target RNAs can be directed by external guide sequences (EGSs) or by the use of the catalytic M1 RNA from E. coli linked to a guide sequence (M1GSs). These are not only basic tools for functional genetic studies in prokaryotic and eukaryotic cells but also promising antibacterial, anticancer and antiviral agents.

Original language	English (US)
Pages (from-to)	212-221
Number of pages	10
Journal	New Biotechnology
Volume	27
Issue number	3
DOIs	https://doi.org/10.1016/j.nbt.2010.03.003
State	Published - Jul 31 2010
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Bioengineering
Molecular Biology

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title = "Inhibition of gene expression by RNase P",

abstract = "The ability to interfere with gene expression is of crucial importance to unravel the function of genes and is also a promising therapeutic strategy. Here we discuss methodologies for inhibition of target RNAs based on the cleavage activity of the essential enzyme, Ribonuclease P (RNase P). RNase P-mediated cleavage of target RNAs can be directed by external guide sequences (EGSs) or by the use of the catalytic M1 RNA from E. coli linked to a guide sequence (M1GSs). These are not only basic tools for functional genetic studies in prokaryotic and eukaryotic cells but also promising antibacterial, anticancer and antiviral agents.",

author = "Lundblad, {Eirik Wasmuth} and Sidney Altman",

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PY - 2010/7/31

Y1 - 2010/7/31

N2 - The ability to interfere with gene expression is of crucial importance to unravel the function of genes and is also a promising therapeutic strategy. Here we discuss methodologies for inhibition of target RNAs based on the cleavage activity of the essential enzyme, Ribonuclease P (RNase P). RNase P-mediated cleavage of target RNAs can be directed by external guide sequences (EGSs) or by the use of the catalytic M1 RNA from E. coli linked to a guide sequence (M1GSs). These are not only basic tools for functional genetic studies in prokaryotic and eukaryotic cells but also promising antibacterial, anticancer and antiviral agents.

AB - The ability to interfere with gene expression is of crucial importance to unravel the function of genes and is also a promising therapeutic strategy. Here we discuss methodologies for inhibition of target RNAs based on the cleavage activity of the essential enzyme, Ribonuclease P (RNase P). RNase P-mediated cleavage of target RNAs can be directed by external guide sequences (EGSs) or by the use of the catalytic M1 RNA from E. coli linked to a guide sequence (M1GSs). These are not only basic tools for functional genetic studies in prokaryotic and eukaryotic cells but also promising antibacterial, anticancer and antiviral agents.

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Inhibition of gene expression by RNase P

Abstract

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