Inhibition of gene expression by RNase P

Eirik Wasmuth Lundblad, Sidney Altman

Research output: Contribution to journalReview article

28 Citations (Scopus)

Abstract

The ability to interfere with gene expression is of crucial importance to unravel the function of genes and is also a promising therapeutic strategy. Here we discuss methodologies for inhibition of target RNAs based on the cleavage activity of the essential enzyme, Ribonuclease P (RNase P). RNase P-mediated cleavage of target RNAs can be directed by external guide sequences (EGSs) or by the use of the catalytic M1 RNA from E. coli linked to a guide sequence (M1GSs). These are not only basic tools for functional genetic studies in prokaryotic and eukaryotic cells but also promising antibacterial, anticancer and antiviral agents.

Original languageEnglish (US)
Pages (from-to)212-221
Number of pages10
JournalNew Biotechnology
Volume27
Issue number3
DOIs
StatePublished - Jul 31 2010
Externally publishedYes

Fingerprint

Ribonuclease P
RNA
Gene expression
RNA Cleavage
Prokaryotic Cells
Gene Expression
Catalytic RNA
Antiviral agents
Eukaryotic Cells
Antineoplastic Agents
Bactericides
Antiviral Agents
Genes
Escherichia coli
Anti-Bacterial Agents
Enzymes
Ribonucleases
Therapeutics
E coli ribonuclease P

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Bioengineering

Cite this

Inhibition of gene expression by RNase P. / Lundblad, Eirik Wasmuth; Altman, Sidney.

In: New Biotechnology, Vol. 27, No. 3, 31.07.2010, p. 212-221.

Research output: Contribution to journalReview article

Lundblad, Eirik Wasmuth ; Altman, Sidney. / Inhibition of gene expression by RNase P. In: New Biotechnology. 2010 ; Vol. 27, No. 3. pp. 212-221.
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