Influence of storage conditions and preservatives on metabolite fingerprints in urine

Xinchen Wang, Haiwei Gu, Susana A. Palma-Duran, Andres Fierro, Paniz Jasbi, Xiaojian Shi, William Bresette, Natasha Tasevska

Research output: Contribution to journalArticle

Abstract

Human urine, which is rich in metabolites, provides valuable approaches for biomarker measurement. Maintaining the stability of metabolites in urine is critical for accurate and reliable research results and subsequent interpretation. In this study, the effect of storage temperature (4, 22, and 40 °C), storage time (24 and 48 h), and use of preservatives (boric acid (BA), thymol) and para-aminobenzoic acid (PABA) on urinary metabolites in the pooled urine samples from 20 participants was systematically investigated using large-scale targeted liquid chromatography tandem mass spectrometry (LC-MS/MS)-based metabolomics. Statistical analysis of 158 reliably detected metabolites showed that metabolites in urine with no preservative remained stable at 4 °C for 24 and 48 h as well as at 22 °C for 24 h, but significant metabolite differences were observed in urine stored at 22 °C for 48 h and at 40 °C. The mere addition of BA caused metabolite changes. Thymol was observed to be effective in maintaining metabolite stability in urine in all the conditions designed, most likely due to the inhibitory effect of thymol on urine microbiota. Our results provide valuable urine preservation guidance during sample storage, which is essential for obtaining reliable, accurate, and reproducible analytical results from urine samples.

Original languageEnglish (US)
Article number203
JournalMetabolites
Volume9
Issue number10
DOIs
StatePublished - Oct 2019

Fingerprint

Dermatoglyphics
Metabolites
Urine
Thymol
4-Aminobenzoic Acid
Liquid chromatography
Biomarkers
Metabolomics
Microbiota
Tandem Mass Spectrometry
Mass spectrometry
Liquid Chromatography
Statistical methods
Temperature
Research

Keywords

  • Mass spectrometry
  • Metabolomics
  • Preservative
  • Temperature
  • Urine storage

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Molecular Biology

Cite this

Wang, X., Gu, H., Palma-Duran, S. A., Fierro, A., Jasbi, P., Shi, X., ... Tasevska, N. (2019). Influence of storage conditions and preservatives on metabolite fingerprints in urine. Metabolites, 9(10), [203]. https://doi.org/10.3390/metabo9100203

Influence of storage conditions and preservatives on metabolite fingerprints in urine. / Wang, Xinchen; Gu, Haiwei; Palma-Duran, Susana A.; Fierro, Andres; Jasbi, Paniz; Shi, Xiaojian; Bresette, William; Tasevska, Natasha.

In: Metabolites, Vol. 9, No. 10, 203, 10.2019.

Research output: Contribution to journalArticle

Wang, X, Gu, H, Palma-Duran, SA, Fierro, A, Jasbi, P, Shi, X, Bresette, W & Tasevska, N 2019, 'Influence of storage conditions and preservatives on metabolite fingerprints in urine', Metabolites, vol. 9, no. 10, 203. https://doi.org/10.3390/metabo9100203
Wang, Xinchen ; Gu, Haiwei ; Palma-Duran, Susana A. ; Fierro, Andres ; Jasbi, Paniz ; Shi, Xiaojian ; Bresette, William ; Tasevska, Natasha. / Influence of storage conditions and preservatives on metabolite fingerprints in urine. In: Metabolites. 2019 ; Vol. 9, No. 10.
@article{5013d6fdb2244924a71d227b40ee3d12,
title = "Influence of storage conditions and preservatives on metabolite fingerprints in urine",
abstract = "Human urine, which is rich in metabolites, provides valuable approaches for biomarker measurement. Maintaining the stability of metabolites in urine is critical for accurate and reliable research results and subsequent interpretation. In this study, the effect of storage temperature (4, 22, and 40 °C), storage time (24 and 48 h), and use of preservatives (boric acid (BA), thymol) and para-aminobenzoic acid (PABA) on urinary metabolites in the pooled urine samples from 20 participants was systematically investigated using large-scale targeted liquid chromatography tandem mass spectrometry (LC-MS/MS)-based metabolomics. Statistical analysis of 158 reliably detected metabolites showed that metabolites in urine with no preservative remained stable at 4 °C for 24 and 48 h as well as at 22 °C for 24 h, but significant metabolite differences were observed in urine stored at 22 °C for 48 h and at 40 °C. The mere addition of BA caused metabolite changes. Thymol was observed to be effective in maintaining metabolite stability in urine in all the conditions designed, most likely due to the inhibitory effect of thymol on urine microbiota. Our results provide valuable urine preservation guidance during sample storage, which is essential for obtaining reliable, accurate, and reproducible analytical results from urine samples.",
keywords = "Mass spectrometry, Metabolomics, Preservative, Temperature, Urine storage",
author = "Xinchen Wang and Haiwei Gu and Palma-Duran, {Susana A.} and Andres Fierro and Paniz Jasbi and Xiaojian Shi and William Bresette and Natasha Tasevska",
year = "2019",
month = "10",
doi = "10.3390/metabo9100203",
language = "English (US)",
volume = "9",
journal = "Metabolites",
issn = "2218-1989",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "10",

}

TY - JOUR

T1 - Influence of storage conditions and preservatives on metabolite fingerprints in urine

AU - Wang, Xinchen

AU - Gu, Haiwei

AU - Palma-Duran, Susana A.

AU - Fierro, Andres

AU - Jasbi, Paniz

AU - Shi, Xiaojian

AU - Bresette, William

AU - Tasevska, Natasha

PY - 2019/10

Y1 - 2019/10

N2 - Human urine, which is rich in metabolites, provides valuable approaches for biomarker measurement. Maintaining the stability of metabolites in urine is critical for accurate and reliable research results and subsequent interpretation. In this study, the effect of storage temperature (4, 22, and 40 °C), storage time (24 and 48 h), and use of preservatives (boric acid (BA), thymol) and para-aminobenzoic acid (PABA) on urinary metabolites in the pooled urine samples from 20 participants was systematically investigated using large-scale targeted liquid chromatography tandem mass spectrometry (LC-MS/MS)-based metabolomics. Statistical analysis of 158 reliably detected metabolites showed that metabolites in urine with no preservative remained stable at 4 °C for 24 and 48 h as well as at 22 °C for 24 h, but significant metabolite differences were observed in urine stored at 22 °C for 48 h and at 40 °C. The mere addition of BA caused metabolite changes. Thymol was observed to be effective in maintaining metabolite stability in urine in all the conditions designed, most likely due to the inhibitory effect of thymol on urine microbiota. Our results provide valuable urine preservation guidance during sample storage, which is essential for obtaining reliable, accurate, and reproducible analytical results from urine samples.

AB - Human urine, which is rich in metabolites, provides valuable approaches for biomarker measurement. Maintaining the stability of metabolites in urine is critical for accurate and reliable research results and subsequent interpretation. In this study, the effect of storage temperature (4, 22, and 40 °C), storage time (24 and 48 h), and use of preservatives (boric acid (BA), thymol) and para-aminobenzoic acid (PABA) on urinary metabolites in the pooled urine samples from 20 participants was systematically investigated using large-scale targeted liquid chromatography tandem mass spectrometry (LC-MS/MS)-based metabolomics. Statistical analysis of 158 reliably detected metabolites showed that metabolites in urine with no preservative remained stable at 4 °C for 24 and 48 h as well as at 22 °C for 24 h, but significant metabolite differences were observed in urine stored at 22 °C for 48 h and at 40 °C. The mere addition of BA caused metabolite changes. Thymol was observed to be effective in maintaining metabolite stability in urine in all the conditions designed, most likely due to the inhibitory effect of thymol on urine microbiota. Our results provide valuable urine preservation guidance during sample storage, which is essential for obtaining reliable, accurate, and reproducible analytical results from urine samples.

KW - Mass spectrometry

KW - Metabolomics

KW - Preservative

KW - Temperature

KW - Urine storage

UR - http://www.scopus.com/inward/record.url?scp=85073495916&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85073495916&partnerID=8YFLogxK

U2 - 10.3390/metabo9100203

DO - 10.3390/metabo9100203

M3 - Article

AN - SCOPUS:85073495916

VL - 9

JO - Metabolites

JF - Metabolites

SN - 2218-1989

IS - 10

M1 - 203

ER -