Existing epidemiological evidence suggests that localized muscle fatigue might be considered as an intrinsic risk factor that causes lack of balance control leading to falls. The goal of the study was to examine how localized muscle fatigue of the knee joint (quadriceps) alters gait parameters that are related to slip propensity. Sixteen healthy young participants were recruited to walk across a vinyl floor surface in two different sessions (Fatigue and No fatigue). Kinematic and kinetic data were collected using a three-dimensional video analysis system and force plates during both sessions. The fatigue session results indicated a substantial increase in heel contact velocity (HCV) and required coefficient of friction (RCOF), as well as a decrease in the transitional acceleration of the whole body COM (TA), walking velocity (WV), and step length (SL). In addition, a positive correlation was observed between RCOF and HCV. These findings provide new insights into the biomechanical relationship between localized muscular fatigue and gait parameters linked with slip propensity. The study concluded that localized muscular fatigue affects gait parameters and hence can be considered as a potential risk factor for slip-induced falls.