Inflammatory effects of Edwardsiella ictaluri lipopolysaccharide modifications in catfish gut

Javier Santander, Jacquelyn Kilbourne, Jie Yeun Park, Taylor Martin, Amanda Loh, Ignacia Diaz, Robert Rojas, Cristopher Segovia, Dale Denardo, Roy Curtiss

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Bacterial lipopolysaccharides (LPS) are structural components of the outer membranes of Gram-negative bacteria and also are potent inducers of inflammation in mammals. Higher vertebrates are extremely sensitive to LPS, but lower vertebrates, like fish, are resistant to their systemic toxic effects. However, the effects of LPS on the fish intestinal mucosa remain unknown. Edwardsiella ictaluri is a primitive member of the Enterobacteriaceae family that causes enteric septicemia in channel catfish (Ictalurus punctatus). E. ictaluri infects and colonizes deep lymphoid tissues upon oral or immersion infection. Both gut and olfactory organs are the primary sites of invasion. At the systemic level, E. ictaluri pathogenesis is relatively well characterized, but our knowledge about E. ictaluri intestinal interaction is limited. Recently, we observed that E. ictaluri oligo-polysaccharide (O-PS) LPS mutants have differential effects on the intestinal epithelia of orally inoculated catfish. Here we evaluate the effects of E. ictaluri O-PS LPS mutants by using a novel catfish intestinal loop model and compare it to the rabbit ileal loop model inoculated with Salmonella enterica serovar Typhimurium LPS. We found evident differences in rabbit ileal loop and catfish ileal loop responses to E. ictaluri and S. Typhimurium LPS. We determined that catfish respond to E. ictaluri LPS but not to S. Typhimurium LPS. We also determined that E. ictaluri inhibits cytokine production and induces disruption of the intestinal fish epithelia in an O-PS-dependent fashion. The E. ictaluri wild type and ΔwibT LPS mutant caused intestinal tissue damage and inhibited proinflammatory cytokine synthesis, in contrast to E. ictaluri Δgne and Δugd LPS mutants. We concluded that the E. ictaluri O-PS subunits play a major role during pathogenesis, since they influence the recognition of the LPS by the intestinal mucosal immune system of the catfish. The LPS structure of E. ictaluri mutants is needed to understand the mechanism of interaction.

Original languageEnglish (US)
Pages (from-to)3394-3404
Number of pages11
JournalInfection and immunity
Volume82
Issue number8
DOIs
StatePublished - 2014

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

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    Santander, J., Kilbourne, J., Park, J. Y., Martin, T., Loh, A., Diaz, I., Rojas, R., Segovia, C., Denardo, D., & Curtiss, R. (2014). Inflammatory effects of Edwardsiella ictaluri lipopolysaccharide modifications in catfish gut. Infection and immunity, 82(8), 3394-3404. https://doi.org/10.1128/IAI.01697-14