Inference of expanded Lrp-like feast/famine transcription factor targets in a non-model organism using protein structure-based prediction

Justin Ashworth, Christopher Plaisier, Fang Yin Lo, David J. Reiss, Nitin S. Baliga

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Widespread microbial genome sequencing presents an opportunity to understand the gene regulatory networks of nonmodel organisms. This requires knowledge of the binding sites for transcription factors whose DNA-binding properties are unknown or difficult to infer. We adapted a protein structure-based method to predict the specificities and putative regulons of homologous transcription factors across diverse species. As a proof-of-concept we predicted the specificities and transcriptional target genes of divergent archaeal feast/famine regulatory proteins, several of which are encoded in the genome of Halobacterium salinarum. This was validated by comparison to experimentally determined specificities for transcription factors in distantly related extremophiles, chromatin immunoprecipitation experiments, and cis-regulatory sequence conservation across eighteen related species of halobacteria. Through this analysis we were able to infer that Halobacterium salinarum employs a divergent local trans-regulatory strategy to regulate genes (carA and carB) involved in arginine and pyrimidine metabolism, whereas Escherichia coli employs an operon. The prediction of gene regulatory binding sites using structure-based methods is useful for the inference of gene regulatory relationships in new species that are otherwise difficult to infer.

Original languageEnglish (US)
Article numbere107863
JournalPLoS One
Volume9
Issue number9
DOIs
StatePublished - Sep 25 2014
Externally publishedYes

Fingerprint

famine
protein structure
Starvation
Halobacterium
Halobacterium salinarum
Transcription Factors
transcription factors
Genes
Regulator Genes
regulator genes
prediction
binding sites
Archaeal Genes
organisms
Euryarchaeota
Microbial Genome
Binding Sites
Regulon
regulon
regulatory sequences

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Inference of expanded Lrp-like feast/famine transcription factor targets in a non-model organism using protein structure-based prediction. / Ashworth, Justin; Plaisier, Christopher; Lo, Fang Yin; Reiss, David J.; Baliga, Nitin S.

In: PLoS One, Vol. 9, No. 9, e107863, 25.09.2014.

Research output: Contribution to journalArticle

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