Induction of lymphoid leukosis transplantable tumours and the establishment of lymphoblastoid cell lines

W. Okazaki, R. L. Witter, C. Romero, K. Nazerian, J. M. Sharma, A. Fadly, D. Ewert

Research output: Contribution to journalArticle

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Abstract

Primary lymphoid tumours induced by the subgroup A, B, C and D lymphoid leukosis viruses RAV-1, RAV-2, RAV-49 and RAV-50, respectively, have been developed as transplantable “B-cell” lymphoid tumours and designated LSCT-RP5, LSCT-RP6, LSCT-RP7 and LSCT-RP8, respectively. There seems little doubt that these are indeed transplants because all produce tumours and death of the inoculated chicks in a very short time (median days to death = 5-10 days), and chromosome analyses reveal that the sex of the tumour cells remains unchanged when passaged in chicks of the opposite sex. We have established 2 in vitro lymphoblastoid lines (LSCC-RP9 and LSCC-RP12) from the RAV-2 transplantable tumour (LSCT-RP6), but were unsuccessful after numerous attempts with RAV-1 transplantable tumours (LSCT-RP5). The LSCC-RP9 and LSCC-RP12 lymphoblastoid cell lines grow rapidly and produce large amounts of virus. These transplantable tumours and lymphoblastoid cell lines should provide valuable materials for studies on virus-host and tumour cell-host inter-relationships.

Original languageEnglish (US)
Pages (from-to)311-329
Number of pages19
JournalAvian Pathology
Volume9
Issue number3
DOIs
StatePublished - Jul 1 1980
Externally publishedYes

Fingerprint

cell lines
Cell Line
neoplasms
Neoplasms
viruses
chicks
death
Viruses
Oncogenic Viruses
Chromosomes, Human, Pair 10
Tumor Cell Line
B-lymphocytes
germ cells
B-Lymphocytes
chromosomes
Transplants
gender
neoplasm cells

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Immunology and Microbiology(all)
  • Food Animals

Cite this

Okazaki, W., Witter, R. L., Romero, C., Nazerian, K., Sharma, J. M., Fadly, A., & Ewert, D. (1980). Induction of lymphoid leukosis transplantable tumours and the establishment of lymphoblastoid cell lines. Avian Pathology, 9(3), 311-329. https://doi.org/10.1080/03079458008418416

Induction of lymphoid leukosis transplantable tumours and the establishment of lymphoblastoid cell lines. / Okazaki, W.; Witter, R. L.; Romero, C.; Nazerian, K.; Sharma, J. M.; Fadly, A.; Ewert, D.

In: Avian Pathology, Vol. 9, No. 3, 01.07.1980, p. 311-329.

Research output: Contribution to journalArticle

Okazaki, W, Witter, RL, Romero, C, Nazerian, K, Sharma, JM, Fadly, A & Ewert, D 1980, 'Induction of lymphoid leukosis transplantable tumours and the establishment of lymphoblastoid cell lines', Avian Pathology, vol. 9, no. 3, pp. 311-329. https://doi.org/10.1080/03079458008418416
Okazaki, W. ; Witter, R. L. ; Romero, C. ; Nazerian, K. ; Sharma, J. M. ; Fadly, A. ; Ewert, D. / Induction of lymphoid leukosis transplantable tumours and the establishment of lymphoblastoid cell lines. In: Avian Pathology. 1980 ; Vol. 9, No. 3. pp. 311-329.
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