TY - JOUR
T1 - Induction of lymphoid leukosis transplantable tumours and the establishment of lymphoblastoid cell lines
AU - Okazaki, W.
AU - Witter, R. L.
AU - Romero, C.
AU - Nazerian, K.
AU - Sharma, J. M.
AU - Fadly, A.
AU - Ewert, D.
PY - 1980/7/1
Y1 - 1980/7/1
N2 - Primary lymphoid tumours induced by the subgroup A, B, C and D lymphoid leukosis viruses RAV-1, RAV-2, RAV-49 and RAV-50, respectively, have been developed as transplantable “B-cell” lymphoid tumours and designated LSCT-RP5, LSCT-RP6, LSCT-RP7 and LSCT-RP8, respectively. There seems little doubt that these are indeed transplants because all produce tumours and death of the inoculated chicks in a very short time (median days to death = 5-10 days), and chromosome analyses reveal that the sex of the tumour cells remains unchanged when passaged in chicks of the opposite sex. We have established 2 in vitro lymphoblastoid lines (LSCC-RP9 and LSCC-RP12) from the RAV-2 transplantable tumour (LSCT-RP6), but were unsuccessful after numerous attempts with RAV-1 transplantable tumours (LSCT-RP5). The LSCC-RP9 and LSCC-RP12 lymphoblastoid cell lines grow rapidly and produce large amounts of virus. These transplantable tumours and lymphoblastoid cell lines should provide valuable materials for studies on virus-host and tumour cell-host inter-relationships.
AB - Primary lymphoid tumours induced by the subgroup A, B, C and D lymphoid leukosis viruses RAV-1, RAV-2, RAV-49 and RAV-50, respectively, have been developed as transplantable “B-cell” lymphoid tumours and designated LSCT-RP5, LSCT-RP6, LSCT-RP7 and LSCT-RP8, respectively. There seems little doubt that these are indeed transplants because all produce tumours and death of the inoculated chicks in a very short time (median days to death = 5-10 days), and chromosome analyses reveal that the sex of the tumour cells remains unchanged when passaged in chicks of the opposite sex. We have established 2 in vitro lymphoblastoid lines (LSCC-RP9 and LSCC-RP12) from the RAV-2 transplantable tumour (LSCT-RP6), but were unsuccessful after numerous attempts with RAV-1 transplantable tumours (LSCT-RP5). The LSCC-RP9 and LSCC-RP12 lymphoblastoid cell lines grow rapidly and produce large amounts of virus. These transplantable tumours and lymphoblastoid cell lines should provide valuable materials for studies on virus-host and tumour cell-host inter-relationships.
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U2 - 10.1080/03079458008418416
DO - 10.1080/03079458008418416
M3 - Article
AN - SCOPUS:0001363401
SN - 0307-9457
VL - 9
SP - 311
EP - 329
JO - Avian Pathology
JF - Avian Pathology
IS - 3
ER -