Induction of 32- and 34-kDa stress proteins by sodium arsenite, heavy metals, and thiol-reactive agents

M. M. Caltabiano, T. P. Koestler, George Poste, R. G. Greig

Research output: Contribution to journalArticle

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Abstract

Challenge of human or murine melanoma cells with sodium arsenite, heavy metals (Zn2+, Cu2+ and Cd2+), or thiol-reactive agents (p-chloromercuribenzoate and iodoacetamide) induced the synthesis of four stress proteins with molecular masses of 100, 90, 72 (a doublet), and 32 (human) or 34 (murine) kDa. Enhanced expression of the 32- and 34-kDa polypeptides (p32 and p34) preceded or paralleled the synthesis of the other stress proteins. Hyperthermia, the calcium ionophore A23187, and amino acid analogs (L-azetidine-2-carboxylic acid and L-canavanine) induced the formation of the major stress proteins, but failed to increase synthesis of p32 and p34. Characterization of the dose and time dependence of p32 and p34 synthesis in human (A375) and murine (B16-F10) melanoma cells, respectively, indicated that these proteins were subject to similar regulatory mechanisms. Electrophoretic analysis of stressed cells pulsed with different metabolic precursors revealed that p32 and p34 were radiolabeled with [35S]methionine or 3H-amino acids but not by [3H]mannose or [35S]cysteine. Polyclonal antibodies raised against human p32 cross-reacted with murine p34. These data suggest that p32 and p34 are closely regulated human and murine gene products, respectively, whose synthesis can be modulated by thiol-reactive reagents. Induction of p32 and p34 by these agents, but not by heat shock, suggests that these proteins are a subset of stress-inducible gene products.

Original languageEnglish (US)
Pages (from-to)13381-13386
Number of pages6
JournalJournal of Biological Chemistry
Volume261
Issue number28
StatePublished - 1986
Externally publishedYes

Fingerprint

Heat-Shock Proteins
Heavy Metals
Sulfhydryl Compounds
Azetidinecarboxylic Acid
Genes
Canavanine
Chloromercuribenzoates
Iodoacetamide
Amino Acids
Sulfhydryl Reagents
Experimental Melanomas
Calcium Ionophores
Calcimycin
Molecular mass
Mannose
Methionine
Cysteine
Melanoma
Fever
sodium arsenite

ASJC Scopus subject areas

  • Biochemistry

Cite this

Induction of 32- and 34-kDa stress proteins by sodium arsenite, heavy metals, and thiol-reactive agents. / Caltabiano, M. M.; Koestler, T. P.; Poste, George; Greig, R. G.

In: Journal of Biological Chemistry, Vol. 261, No. 28, 1986, p. 13381-13386.

Research output: Contribution to journalArticle

Caltabiano, M. M. ; Koestler, T. P. ; Poste, George ; Greig, R. G. / Induction of 32- and 34-kDa stress proteins by sodium arsenite, heavy metals, and thiol-reactive agents. In: Journal of Biological Chemistry. 1986 ; Vol. 261, No. 28. pp. 13381-13386.
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