TY - JOUR
T1 - Incidence and characterization of Potato virus V infections in Iran
AU - Shamsadden-Saeed, Fatemeh
AU - Massumi, Hossain
AU - Moradi, Sakineh
AU - Maddahian, Mohammad
AU - Heydarnejad, Jahangir
AU - Pour, Akbar Hosseini
AU - Varsani, Arvind
N1 - Publisher Copyright:
© 2013, Indian Virological Society.
PY - 2014/1/9
Y1 - 2014/1/9
N2 - A survey was carried out to determine the extent of Potato virus V (PVV) infection, together with other potyviruses, in Iran in both commercial and local potato varieties. We found a low incidence of PVV in commercial varieties compared to a local potato cultivar Zardi, in which we noted a PVV infection up to ~32.9 %. We determined the genomic sequence 9,812 nucleotides of one isolate (KER.LAL.P) from cultivar Agria and the 3′-terminal sequence including coat protein (CP) gene of four additional isolates from cultivar Zardi. The Iranian isolate PVV KER.LAL.P was found to share 91 % sequence identity with the Scottish isolate DV-42 (AJ243766) of PVV. The CP gene sequences of the PVV isolates from Iran shared 96.5 to 99.3 % pairwise nucleotide identity and they shared <97.5 % pairwise identity with the CP sequences of all other PVV isolates available in public databases. Our host studies indicated that the Iranian PVV isolate had a narrower host range and infected Nicotiana debneyi and N. glutinosa test plants. Within the commercial varieties of potato in Iran, we noted a significant amount of mixed potyvirus infection. This study is the first report of occurrence and complete genome of PVV in Iran.
AB - A survey was carried out to determine the extent of Potato virus V (PVV) infection, together with other potyviruses, in Iran in both commercial and local potato varieties. We found a low incidence of PVV in commercial varieties compared to a local potato cultivar Zardi, in which we noted a PVV infection up to ~32.9 %. We determined the genomic sequence 9,812 nucleotides of one isolate (KER.LAL.P) from cultivar Agria and the 3′-terminal sequence including coat protein (CP) gene of four additional isolates from cultivar Zardi. The Iranian isolate PVV KER.LAL.P was found to share 91 % sequence identity with the Scottish isolate DV-42 (AJ243766) of PVV. The CP gene sequences of the PVV isolates from Iran shared 96.5 to 99.3 % pairwise nucleotide identity and they shared <97.5 % pairwise identity with the CP sequences of all other PVV isolates available in public databases. Our host studies indicated that the Iranian PVV isolate had a narrower host range and infected Nicotiana debneyi and N. glutinosa test plants. Within the commercial varieties of potato in Iran, we noted a significant amount of mixed potyvirus infection. This study is the first report of occurrence and complete genome of PVV in Iran.
KW - Complete genome
KW - Iran
KW - Phylogenetic analysis
KW - Potato virus V
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U2 - 10.1007/s13337-013-0178-4
DO - 10.1007/s13337-013-0178-4
M3 - Article
AN - SCOPUS:84941091743
SN - 2347-3584
VL - 25
SP - 78
EP - 84
JO - VirusDisease
JF - VirusDisease
IS - 1
ER -