Abstract
Protein crystallization in cells has been observed several times in nature. However, owing to their small size these crystals have not yet been used for X-ray crystallographic analysis. We prepared nano-sized in vivo-grown crystals of Trypanosoma brucei enzymes and applied the emerging method of free-electron laser-based serial femtosecond crystallography to record interpretable diffraction data. This combined approach will open new opportunities in structural systems biology.
Original language | English (US) |
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Pages (from-to) | 259-262 |
Number of pages | 4 |
Journal | Nature Methods |
Volume | 9 |
Issue number | 3 |
DOIs | |
State | Published - Mar 2012 |
ASJC Scopus subject areas
- Biotechnology
- Biochemistry
- Molecular Biology
- Cell Biology
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Crystal structure of Cathepsin B from Trypanosoma Brucei
Koopmann, R. (Contributor), Cupelli, K. (Contributor), Redecke, L. (Contributor), Nass, K. (Contributor), Deponte, D. P. (Contributor), White, T. A. (Contributor), Stellato, F. (Contributor), Rehders, D. (Contributor), Liang, M. (Contributor), Andreasson, J. (Contributor), Aquila, A. (Contributor), Bajt, S. (Contributor), Barthelmess, M. (Contributor), Barty, A. (Contributor), Bogan, M. J. (Contributor), Bostedt, C. (Contributor), Boutet, S. (Contributor), Bozek, J. D. (Contributor), Caleman, C. (Contributor), Coppola, N. (Contributor), Davidsson, J. (Contributor), Doak, R. B. (Contributor), Ekeberg, T. (Contributor), Epp, S. W. (Contributor), Erk, B. (Contributor), Fleckenstein, H. (Contributor), Foucar, L. (Contributor), Graafsma, H. (Contributor), Gumprecht, L. (Contributor), Hajdu, J. (Contributor), Hampton, C. Y. (Contributor), Hartmann, A. (Contributor), Hartmann, R. (Contributor), Hauser, G. (Contributor), Hirsemann, H. (Contributor), Holl, P. (Contributor), Hunter, M. S. (Contributor), Kassemeyer, S. (Contributor), Kirian, R. (Contributor), Lomb, L. (Contributor), Maia, F. R. N. C. (Contributor), Kimmel, N. (Contributor), Martin, A. V. (Contributor), Messerschmidt, M. (Contributor), Reich, C. (Contributor), Rolles, D. (Contributor), Rudek, B. (Contributor), Rudenko, A. (Contributor), Schlichting, I. (Contributor), Schulz, J. (Contributor), Seibert, M. M. (Contributor), Shoeman, R. L. (Contributor), Sierra, R. G. (Contributor), Soltau, H. (Contributor), Stern, S. (Contributor), Strülder, L. (Contributor), Timneanu, N. (Contributor), Ullrich, J. (Contributor), Wang, X. (Contributor), Weidenspointner, G. (Contributor), Weierstall, U. (Contributor), Williams, G. J. (Contributor), Wunderer, C. B. (Contributor), Fromme, P. (Contributor), Spence, J. C. H. (Contributor), Stehle, T. (Contributor), Chapman, H. N. (Contributor), Betzel, C. (Contributor) & Duszenko, M. (Contributor), Protein Data Bank (PDB), Nov 2 2011
DOI: 10.2210/pdb3MOR, https://www.wwpdb.org/pdb?id=pdb_00003mor
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