TY - JOUR
T1 - In vivo excision, cloning, and broad-host-range transfer of large bacterial DNA segments using VEX-Capture.
AU - Wilson, James W.
AU - Nickerson, Cheryl
PY - 2007
Y1 - 2007
N2 - The performance of many bacterial genetic experiments would benefit from a convenient method to clone large sets of genes (20-100+ kb) and transfer these genes to a wide range of other bacterial recipients. The VEX-Capture technique allows such large genomic segments to be cloned in vivo onto a broad-host-range IncP plasmid that is able to self-transfer to a wide variety of Gram-negative bacteria. The advantages of VEX-Capture are its efficiency, specificity, and use of common molecular biological techniques that do not require non-standard equipment and are easily applicable to many types of bacterial species. Here, we describe the VEX-Capture experimental protocol using Salmonella typhimurium as the source of the target DNA segment.
AB - The performance of many bacterial genetic experiments would benefit from a convenient method to clone large sets of genes (20-100+ kb) and transfer these genes to a wide range of other bacterial recipients. The VEX-Capture technique allows such large genomic segments to be cloned in vivo onto a broad-host-range IncP plasmid that is able to self-transfer to a wide variety of Gram-negative bacteria. The advantages of VEX-Capture are its efficiency, specificity, and use of common molecular biological techniques that do not require non-standard equipment and are easily applicable to many types of bacterial species. Here, we describe the VEX-Capture experimental protocol using Salmonella typhimurium as the source of the target DNA segment.
UR - http://www.scopus.com/inward/record.url?scp=42149188971&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=42149188971&partnerID=8YFLogxK
U2 - 10.1007/978-1-59745-512-1_7
DO - 10.1007/978-1-59745-512-1_7
M3 - Article
C2 - 18363234
AN - SCOPUS:42149188971
SN - 1064-3745
VL - 394
SP - 105
EP - 118
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -