In Situ Analysis of Membrane-Protein Binding Kinetics and Cell–Surface Adhesion Using Plasmonic Scattering Microscopy

Pengfei Zhang, Xinyu Zhou, Jiapei Jiang, Jayeeta Kolay, Rui Wang, Guangzhong Ma, Zijian Wan, Shaopeng Wang

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Surface plasmon resonance microscopy (SPRM) is an excellent platform for in situ studying cell-substrate interactions. However, SPRM suffers from poor spatial resolution and small field of view. Herein, we demonstrate plasmonic scattering microscopy (PSM) by adding a dry objective on a popular prism-coupled surface plasmon resonance (SPR) system. PSM not only retains SPRM's high sensitivity and real-time analysis capability, but also provides ≈7 times higher spatial resolution and ≈70 times larger field of view than the typical SPRM, thus providing more details about membrane protein response to ligand binding on over 100 cells simultaneously. In addition, PSM allows quantifying the target movements in the axial direction with a high spatial resolution, thus allowing mapping adhesion spring constants for quantitatively describing the mechanical properties of the cell-substrate contacts. This work may offer a powerful and cost-effective strategy for upgrading current SPR products.

Original languageEnglish (US)
Article numbere202209469
JournalAngewandte Chemie - International Edition
Volume61
Issue number42
DOIs
StatePublished - Oct 17 2022
Externally publishedYes

Keywords

  • Biosensors
  • Cell Adhesion
  • Drug Discovery
  • Membrane Proteins
  • Surface Plasmon Resonance

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

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