Immunogenicity in humans of a recombinant bacterial antigen delivered in a transgenic potato

Carol O. Tacket, Hugh Mason, Genevieve Losonsky, John D. Clements, Myron M. Levine, Charles J. Arntzen

Research output: Contribution to journalArticle

456 Citations (Scopus)

Abstract

Compared with vaccine delivery by injection, oral vaccines offer the hope of more convenient immunization strategies and a more practical means of implementing universal vaccination programs throughout the world. Oral vaccines act by stimulatIng the immune system at effector sites (lymphoid tissue) located in the gut. Genetic engineering has been used with variable success to design living and non-living systems as a means to deliver antigens to these sites and to stimulate a desired immune response. More recently, plant biotechnology techniques have been used to create plants which contain a gene derived from a human pathogen; the resultant plant tissues will accumulate an antigenic protein encoded by the foreign DNA. In pre-clinical trials, we found that antigenic proteins produced in transgenic plants retained immunogenic properties when purified; if injected into mice the antigen caused production of protein-specific antibodies. Moreover, in some experiments, if the plant tissues were simply fed to mice, a mucosal immune response occurred. The present study was conducted as a proof of principle to determine if humans would also develop a serum and/or mucosal immune response to an antigen delivered in an uncooked foodstuff.

Original languageEnglish (US)
Pages (from-to)607-609
Number of pages3
JournalNature Medicine
Volume4
Issue number5
DOIs
StatePublished - 1998
Externally publishedYes

Fingerprint

Bacterial Antigens
Solanum tuberosum
Vaccines
Tissue
Antigens
Mucosal Immunity
Immunization
Genetic engineering
Proteins
Immune system
Pathogens
Biotechnology
Genetic Engineering
Genetically Modified Plants
Histocompatibility Antigens Class II
Lymphoid Tissue
Genes
Immune System
Vaccination
Antibodies

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Tacket, C. O., Mason, H., Losonsky, G., Clements, J. D., Levine, M. M., & Arntzen, C. J. (1998). Immunogenicity in humans of a recombinant bacterial antigen delivered in a transgenic potato. Nature Medicine, 4(5), 607-609. https://doi.org/10.1038/nm0598-607

Immunogenicity in humans of a recombinant bacterial antigen delivered in a transgenic potato. / Tacket, Carol O.; Mason, Hugh; Losonsky, Genevieve; Clements, John D.; Levine, Myron M.; Arntzen, Charles J.

In: Nature Medicine, Vol. 4, No. 5, 1998, p. 607-609.

Research output: Contribution to journalArticle

Tacket, CO, Mason, H, Losonsky, G, Clements, JD, Levine, MM & Arntzen, CJ 1998, 'Immunogenicity in humans of a recombinant bacterial antigen delivered in a transgenic potato', Nature Medicine, vol. 4, no. 5, pp. 607-609. https://doi.org/10.1038/nm0598-607
Tacket, Carol O. ; Mason, Hugh ; Losonsky, Genevieve ; Clements, John D. ; Levine, Myron M. ; Arntzen, Charles J. / Immunogenicity in humans of a recombinant bacterial antigen delivered in a transgenic potato. In: Nature Medicine. 1998 ; Vol. 4, No. 5. pp. 607-609.
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