Imaging single cells in a beam of live cyanobacteria with an X-ray laser

Gijs Van Der Schot; Martin Svenda; Filipe R.N.C. Maia; Max Hantke; Daniel P. Deponte; M. Marvin Seibert; Andrew Aquila; Joachim Schulz; Richard Kirian; Mengning Liang; Francesco Stellato; Bianca Iwan; Jakob Andreasson; Nicusor Timneanu; Daniel Westphal; F. Nunes Almeida; Dusko Odic; Dirk Hasse; Gunilla H. Carlsson; Daniel S.D. Larsson; Anton Barty; Andrew V. Martin; Sebastian Schorb; Christoph Bostedt; John D. Bozek; Daniel Rolles; Artem Rudenko; Sascha Epp; Lutz Foucar; Benedikt Rudek; Robert Hartmann; Nils Kimmel; Peter Holl; Lars Englert; Ne Te Duane Loh; Henry N. Chapman; Inger Andersson; Janos Hajdu; Tomas Ekeberg

doi:10.1038/ncomms6704

Imaging single cells in a beam of live cyanobacteria with an X-ray laser

Gijs Van Der Schot, Martin Svenda, Filipe R.N.C. Maia, Max Hantke, Daniel P. Deponte, M. Marvin Seibert, Andrew Aquila, Joachim Schulz, Richard Kirian, Mengning Liang, Francesco Stellato, Bianca Iwan, Jakob Andreasson, Nicusor Timneanu, Daniel Westphal, F. Nunes Almeida, Dusko Odic, Dirk Hasse, Gunilla H. Carlsson, Daniel S.D. LarssonAnton Barty, Andrew V. Martin, Sebastian Schorb, Christoph Bostedt, John D. Bozek, Daniel Rolles, Artem Rudenko, Sascha Epp, Lutz Foucar, Benedikt Rudek, Robert Hartmann, Nils Kimmel, Peter Holl, Lars Englert, Ne Te Duane Loh, Henry N. Chapman, Inger Andersson, Janos Hajdu, Tomas Ekeberg

Research output: Contribution to journal › Article › peer-review

144 Scopus citations

Abstract

There exists a conspicuous gap of knowledge about the organization of life at mesoscopic levels. Ultra-fast coherent diffractive imaging with X-ray free-electron lasers can probe structures at the relevant length scales and may reach sub-nanometer resolution on micron-sized living cells. Here we show that we can introduce a beam of aerosolised cyanobacteria into the focus of the Linac Coherent Light Source and record diffraction patterns from individual living cells at very low noise levels and at high hit ratios. We obtain two-dimensional projection images directly from the diffraction patterns, and present the results as synthetic X-ray Nomarski images calculated from the complex-valued reconstructions. We further demonstrate that it is possible to record diffraction data to nanometer resolution on live cells with X-ray lasers. Extension to sub-nanometer resolution is within reach, although improvements in pulse parameters and X-ray area detectors will be necessary to unlock this potential.

Original language	English (US)
Article number	5704
Journal	Nature communications
Volume	6
DOIs	https://doi.org/10.1038/ncomms6704
State	Published - Feb 2015
Externally published	Yes

ASJC Scopus subject areas

General Chemistry
General Biochemistry, Genetics and Molecular Biology
General Physics and Astronomy

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Van Der Schot, G., Svenda, M., Maia, F. R. N. C., Hantke, M., Deponte, D. P., Seibert, M. M., Aquila, A., Schulz, J., Kirian, R., Liang, M., Stellato, F., Iwan, B., Andreasson, J., Timneanu, N., Westphal, D., Almeida, F. N., Odic, D., Hasse, D., Carlsson, G. H., ... Ekeberg, T. (2015). Imaging single cells in a beam of live cyanobacteria with an X-ray laser. Nature communications, 6, Article 5704. https://doi.org/10.1038/ncomms6704

Van Der Schot, G, Svenda, M, Maia, FRNC, Hantke, M, Deponte, DP, Seibert, MM, Aquila, A, Schulz, J, Kirian, R, Liang, M, Stellato, F, Iwan, B, Andreasson, J, Timneanu, N, Westphal, D, Almeida, FN, Odic, D, Hasse, D, Carlsson, GH, Larsson, DSD, Barty, A, Martin, AV, Schorb, S, Bostedt, C, Bozek, JD, Rolles, D, Rudenko, A, Epp, S, Foucar, L, Rudek, B, Hartmann, R, Kimmel, N, Holl, P, Englert, L, Duane Loh, NT, Chapman, HN, Andersson, I, Hajdu, J & Ekeberg, T 2015, 'Imaging single cells in a beam of live cyanobacteria with an X-ray laser', Nature communications, vol. 6, 5704. https://doi.org/10.1038/ncomms6704

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title = "Imaging single cells in a beam of live cyanobacteria with an X-ray laser",

abstract = "There exists a conspicuous gap of knowledge about the organization of life at mesoscopic levels. Ultra-fast coherent diffractive imaging with X-ray free-electron lasers can probe structures at the relevant length scales and may reach sub-nanometer resolution on micron-sized living cells. Here we show that we can introduce a beam of aerosolised cyanobacteria into the focus of the Linac Coherent Light Source and record diffraction patterns from individual living cells at very low noise levels and at high hit ratios. We obtain two-dimensional projection images directly from the diffraction patterns, and present the results as synthetic X-ray Nomarski images calculated from the complex-valued reconstructions. We further demonstrate that it is possible to record diffraction data to nanometer resolution on live cells with X-ray lasers. Extension to sub-nanometer resolution is within reach, although improvements in pulse parameters and X-ray area detectors will be necessary to unlock this potential.",

author = "{Van Der Schot}, Gijs and Martin Svenda and Maia, {Filipe R.N.C.} and Max Hantke and Deponte, {Daniel P.} and Seibert, {M. Marvin} and Andrew Aquila and Joachim Schulz and Richard Kirian and Mengning Liang and Francesco Stellato and Bianca Iwan and Jakob Andreasson and Nicusor Timneanu and Daniel Westphal and Almeida, {F. Nunes} and Dusko Odic and Dirk Hasse and Carlsson, {Gunilla H.} and Larsson, {Daniel S.D.} and Anton Barty and Martin, {Andrew V.} and Sebastian Schorb and Christoph Bostedt and Bozek, {John D.} and Daniel Rolles and Artem Rudenko and Sascha Epp and Lutz Foucar and Benedikt Rudek and Robert Hartmann and Nils Kimmel and Peter Holl and Lars Englert and {Duane Loh}, {Ne Te} and Chapman, {Henry N.} and Inger Andersson and Janos Hajdu and Tomas Ekeberg",

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doi = "10.1038/ncomms6704",

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AU - Van Der Schot, Gijs

AU - Svenda, Martin

AU - Maia, Filipe R.N.C.

AU - Hantke, Max

AU - Deponte, Daniel P.

AU - Seibert, M. Marvin

AU - Aquila, Andrew

AU - Schulz, Joachim

AU - Kirian, Richard

AU - Liang, Mengning

AU - Stellato, Francesco

AU - Iwan, Bianca

AU - Andreasson, Jakob

AU - Timneanu, Nicusor

AU - Westphal, Daniel

AU - Almeida, F. Nunes

AU - Odic, Dusko

AU - Hasse, Dirk

AU - Carlsson, Gunilla H.

AU - Larsson, Daniel S.D.

AU - Barty, Anton

AU - Martin, Andrew V.

AU - Schorb, Sebastian

AU - Bostedt, Christoph

AU - Bozek, John D.

AU - Rolles, Daniel

AU - Rudenko, Artem

AU - Epp, Sascha

AU - Foucar, Lutz

AU - Rudek, Benedikt

AU - Hartmann, Robert

AU - Kimmel, Nils

AU - Holl, Peter

AU - Englert, Lars

AU - Duane Loh, Ne Te

AU - Chapman, Henry N.

AU - Andersson, Inger

AU - Hajdu, Janos

AU - Ekeberg, Tomas

PY - 2015/2

Y1 - 2015/2

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AB - There exists a conspicuous gap of knowledge about the organization of life at mesoscopic levels. Ultra-fast coherent diffractive imaging with X-ray free-electron lasers can probe structures at the relevant length scales and may reach sub-nanometer resolution on micron-sized living cells. Here we show that we can introduce a beam of aerosolised cyanobacteria into the focus of the Linac Coherent Light Source and record diffraction patterns from individual living cells at very low noise levels and at high hit ratios. We obtain two-dimensional projection images directly from the diffraction patterns, and present the results as synthetic X-ray Nomarski images calculated from the complex-valued reconstructions. We further demonstrate that it is possible to record diffraction data to nanometer resolution on live cells with X-ray lasers. Extension to sub-nanometer resolution is within reach, although improvements in pulse parameters and X-ray area detectors will be necessary to unlock this potential.

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Imaging single cells in a beam of live cyanobacteria with an X-ray laser

Abstract

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