Abstract

A substantial portion of the atmospheric particle budget is of biological origin (human and animal dander, plant and insect debris, etc.). These bioaerosols can be considered information-rich packets of biochemical data specific to the organism of origin. In this study, bioaerosol samples from various indoor environments were analyzed to create identifiable patterns attributable to a source level of occupation. Air samples were collected from environments representative of human high-traffic- and low-traffic indoor spaces along with direct human skin sampling. In all settings, total suspended particulate matter was collected and the total aerosol protein concentration ranged from 0.03 to 1.2 μg/m3. High performance liquid chromatography was chosen as a standard analysis technique for the examination of aqueous aerosol extracts to distinguish signatures of occupation compared to environmental background. The results of this study suggest that bioaerosol "fingerprinting" is possible with the two test environments being distinguishable at a 97 % confidence interval. [Figure not available: see fulltext.]

Original languageEnglish (US)
Pages (from-to)351-357
Number of pages7
JournalAnalytical and Bioanalytical Chemistry
Volume405
Issue number1
DOIs
StatePublished - Jan 2013

Fingerprint

Aerosols
High Pressure Liquid Chromatography
Occupations
Particulate Matter
High performance liquid chromatography
Dander
Debris
Skin
Animals
Budgets
Sampling
Insects
Air
Confidence Intervals
Proteins

Keywords

  • Bioaerosols
  • Environmental monitoring
  • Environmental pattern recognition
  • HPLC
  • Separation

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

Cite this

Identifying indoor environmental patterns from bioaerosol material using HPLC. / Staton, Sarah J R; Castillo, Josemar A.; Taylor, Thomas; Herckes, Pierre; Hayes, Mark.

In: Analytical and Bioanalytical Chemistry, Vol. 405, No. 1, 01.2013, p. 351-357.

Research output: Contribution to journalArticle

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N2 - A substantial portion of the atmospheric particle budget is of biological origin (human and animal dander, plant and insect debris, etc.). These bioaerosols can be considered information-rich packets of biochemical data specific to the organism of origin. In this study, bioaerosol samples from various indoor environments were analyzed to create identifiable patterns attributable to a source level of occupation. Air samples were collected from environments representative of human high-traffic- and low-traffic indoor spaces along with direct human skin sampling. In all settings, total suspended particulate matter was collected and the total aerosol protein concentration ranged from 0.03 to 1.2 μg/m3. High performance liquid chromatography was chosen as a standard analysis technique for the examination of aqueous aerosol extracts to distinguish signatures of occupation compared to environmental background. The results of this study suggest that bioaerosol "fingerprinting" is possible with the two test environments being distinguishable at a 97 % confidence interval. [Figure not available: see fulltext.]

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