Identification of the promoter region for the colanic acid polysaccharide biosynthetic genes in Escherichia coli K-12

Valerie Stout

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

The colanic acid polysaccharide capsule biosynthetic genes (cps genes) are primarily clustered at one site located at about 45 min on the Escherichia coli chromosome. The network of proteins involved in regulating the expression of these genes includes the two positive regulators RcsA and RcsB. This work describes the site of action of these two activator proteins and the promoter of the cps genes. It is likely that the cps genes are arranged in a single long operon that is at least 13.5 kb. The promoter region was identified with fusions to lacZ that resulted in regulated expression by the Res network of regulatory proteins, and the start site of transcription was identified by primer extension. The operator region was cloned from Kohara phage to multicopy plasmids and identified by titrating RcsA or RcsB. Sequence analysis of the promoter and operator region revealed homology to the JUMP start element found in the untranslated region of many exopolysaccharide biosynthetic operons. In addition, the deduced amino acid sequence of the amino terminus of the first open reading frame of the cps operon was found to be homologous to proteins encoded by the exopolysaccharide biosynthetic operons of Klebsiella pneumoniae and Erwinia amylovora.

Original languageEnglish (US)
Pages (from-to)4273-4280
Number of pages8
JournalJournal of Bacteriology
Volume178
Issue number14
StatePublished - 1996

Fingerprint

Operon
Genetic Promoter Regions
Polysaccharides
Escherichia coli
Capsules
Genes
Proteins
Erwinia amylovora
Untranslated Regions
Transcription Initiation Site
Klebsiella pneumoniae
Workplace
Bacteriophages
Open Reading Frames
Sequence Analysis
Amino Acid Sequence
Plasmids
Chromosomes
Gene Expression
colanic acid

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Identification of the promoter region for the colanic acid polysaccharide biosynthetic genes in Escherichia coli K-12. / Stout, Valerie.

In: Journal of Bacteriology, Vol. 178, No. 14, 1996, p. 4273-4280.

Research output: Contribution to journalArticle

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