Identification of a role for CLASP2 in insulin action

Paul Langlais, James L. Dillon, April Mengos, Debra Baluch, Ranna Ardebili, Danielle N. Miranda, Xitao Xie, Bradlee L. Heckmann, Jun Liu, Lawrence J. Mandarino

Research output: Contribution to journalArticle

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Abstract

Insulin stimulates the mobilization of glucose transporter 4 (GLUT4) storage vesicles to the plasma membrane, resulting in an influx of glucose into target tissues such as muscle and fat. Wepresent evidence that CLIP-associating protein 2 (CLASP2), a protein previously unassociated with insulin action, is responsive to insulin stimulation. Using mass spectrometry-based protein identification combined with phosphoantibody immunoprecipitation in L6 myotubes, we detected a 4.8-fold increase of CLASP2 in the anti-phosphoserine immunoprecipitates upon insulin stimulation. Western blotting of CLASP2 immunoprecipitates with the phosphoantibody confirmed the finding that CLASP2 undergoes insulin-stimulated phosphorylation, and a number of novel phosphorylation sites were identified. Confocal imaging of L6 myotubes revealed that CLASP2 colocalizes with GLUT4 at the plasma membrane within areas of insulin-mediated cortical actin remodeling. CLASP2 is responsible for directing the distal end of microtubules to the cell cortex, and it has been shown thatGLUT4travels along microtubule tracks. In support of the concept that CLASP2 plays a role in the trafficking of GLUT4 at the cell periphery, CLASP2 knockdown by siRNA in L6 myotubes interfered with insulin-stimulated GLUT4 localization to the plasma membrane. Furthermore, siRNA mediated knockdown of CLASP2 in 3T3-L1 adipocytes inhibited insulin-stimulated glucose transport. We therefore propose a new model for CLASP2 in insulin action, where CLASP2 directs the delivery of GLUT4 to cell cortex landing zones important for insulin action.

Original languageEnglish (US)
Pages (from-to)39245-39253
Number of pages9
JournalJournal of Biological Chemistry
Volume287
Issue number46
DOIs
StatePublished - Nov 9 2012

Fingerprint

Insulin
Facilitative Glucose Transport Proteins
Proteins
Skeletal Muscle Fibers
Cell membranes
Phosphorylation
Cell Membrane
Microtubules
Small Interfering RNA
Phosphoserine
Glucose
Landing
Immunoprecipitation
Adipocytes
Mass spectrometry
Muscle
Actins
Mass Spectrometry
Western Blotting
Fats

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Langlais, P., Dillon, J. L., Mengos, A., Baluch, D., Ardebili, R., Miranda, D. N., ... Mandarino, L. J. (2012). Identification of a role for CLASP2 in insulin action. Journal of Biological Chemistry, 287(46), 39245-39253. https://doi.org/10.1074/jbc.M112.394148

Identification of a role for CLASP2 in insulin action. / Langlais, Paul; Dillon, James L.; Mengos, April; Baluch, Debra; Ardebili, Ranna; Miranda, Danielle N.; Xie, Xitao; Heckmann, Bradlee L.; Liu, Jun; Mandarino, Lawrence J.

In: Journal of Biological Chemistry, Vol. 287, No. 46, 09.11.2012, p. 39245-39253.

Research output: Contribution to journalArticle

Langlais, P, Dillon, JL, Mengos, A, Baluch, D, Ardebili, R, Miranda, DN, Xie, X, Heckmann, BL, Liu, J & Mandarino, LJ 2012, 'Identification of a role for CLASP2 in insulin action', Journal of Biological Chemistry, vol. 287, no. 46, pp. 39245-39253. https://doi.org/10.1074/jbc.M112.394148
Langlais P, Dillon JL, Mengos A, Baluch D, Ardebili R, Miranda DN et al. Identification of a role for CLASP2 in insulin action. Journal of Biological Chemistry. 2012 Nov 9;287(46):39245-39253. https://doi.org/10.1074/jbc.M112.394148
Langlais, Paul ; Dillon, James L. ; Mengos, April ; Baluch, Debra ; Ardebili, Ranna ; Miranda, Danielle N. ; Xie, Xitao ; Heckmann, Bradlee L. ; Liu, Jun ; Mandarino, Lawrence J. / Identification of a role for CLASP2 in insulin action. In: Journal of Biological Chemistry. 2012 ; Vol. 287, No. 46. pp. 39245-39253.
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