Identification and nucleotide sequence of the thymidine kinase gene of Shope fibroma virus

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Abstract

The thymidine kinase (TK) gene of Shope fibroma virus (SFV), a tumorigenic leporipoxvirus, was localized within the viral genome with degenerate oligonucleotide probes. These probes were constructed to two regions of high sequence conservation between the vaccinia virus TK gene and those of several known eucaryotic cellular TK genes, including human, mouse, hamster, and chicken TK genes. The oligonucleotide probes initially localized the SFV TK gene 50 kilobase (kb) from the right terminus of the 160-kb SFV genome within the 9.5-kb BamHI-HindIII fragment E. Fine-mapping analysis indicated that the TK gene was within a 1.2-kb AvaI-HaeIII fragment, and DNA sequencing of this region revealed an open reading frame capable of encoding a polypeptide of 176 amino acids possessing considerable homology to the TK genes of the vaccinia, variola, and monkeypox orthopoxviruses and also to a variety of cellular TK genes. Homology matrix analysis and homology scores suggest that the SFV TK gene has diverged significantly from its counterpart members in the orthopoxvirus genus. Nevertheless, the presence of conserved upstream open reading frames on the 5' side of all of the poxvirus TK genes indicates a similarity of functional organization between the orthopoxviruses and leporipoxviruses. These data suggest a common ancestral origin for at least some of the unique internal regions of the leporipoxviruses and orthopoxviruses as exemplified by SFV and vaccinia virus, respectively.

Original languageEnglish (US)
Pages (from-to)920-927
Number of pages8
JournalJournal of Virology
Volume60
Issue number3
StatePublished - Dec 1 1986
Externally publishedYes

Fingerprint

Rabbit fibroma virus
Rabbit Fibroma Virus
thymidine kinase
Thymidine Kinase
nucleotide sequences
Orthopoxvirus
Leporipoxvirus
Genes
genes
Vaccinia virus
oligonucleotide probes
Oligonucleotide Probes
Open Reading Frames
open reading frames
Monkeypox
Poxviridae
Vaccinia
Smallpox
genome
Vaccinium

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Identification and nucleotide sequence of the thymidine kinase gene of Shope fibroma virus. / Upton, C.; McFadden, Douglas.

In: Journal of Virology, Vol. 60, No. 3, 01.12.1986, p. 920-927.

Research output: Contribution to journalArticle

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