Identification and characterization of a new gene of Escherichia coli K-12 involved in outer membrane permeability.

B. A. Sampson, R. Misra, S. A. Benson

Research output: Contribution to journalArticle

157 Scopus citations

Abstract

Using a genetic selection for mutations which allow large maltodextrins to cross the outer membrane of Escherichia coli in the absence of the LamB maltoporin, we have obtained and characterized two mutations that define a new locus of E. coli. We have designated this locus imp for increased membrane permeability. Mapping studies show that the imp gene resides at approximately 1.2 min on the E. coli chromosome. The mutations alter the permeability of the outer membrane resulting in increased sensitivity to detergents, antibiotics and dyes. The mutations are nonreverting and codominant. Genetic analysis of the mutations suggest that the imp gene is an essential gene. We describe a general cloning strategy that can be used to clone both dominant and recessive alleles. Using this technique, we have cloned the wild-type and mutant imp alleles onto a low copy number plasmid.

Original languageEnglish (US)
Pages (from-to)491-501
Number of pages11
JournalGenetics
Volume122
Issue number3
StatePublished - Jul 1989
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

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