Human RNase P ribonucleoprotein is required for formation of initiation complexes of RNA polymerase III

Raphael Serruya, Natalie Orlovetskie, Robert Reiner, Yana Dehtiar-Zilber, Donna Wesolowski, Sidney Altman, Nayef Jarrous

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11 Scopus citations

Abstract

Human RNase P is implicated in transcription of small non-coding RNA genes by RNA polymerase III (Pol III), but the precise role of this ribonucleoprotein therein remains unknown. We here show that targeted destruction of HeLa nuclear RNase P inhibits transcription of 5S rRNA genes in whole cell extracts, if this precedes the stage of initiation complex formation. Biochemical purification analyses further reveal that this ribonucleoprotein is recruited to 5S rRNA genes as a part of proficient initiation complexes and the activity persists at reinitiation. Knockdown of RNase P abolishes the assembly of initiation complexes by preventing the formation of the initiation sub-complex of Pol III. Our results demonstrate that the structural intactness, but not the endoribonucleolytic activity per se, of RNase P is critical for the function of Pol III in cells and in extracts.

Original languageEnglish (US)
Pages (from-to)5442-5450
Number of pages9
JournalNucleic acids research
Volume43
Issue number11
DOIs
StatePublished - Apr 24 2015

ASJC Scopus subject areas

  • Genetics

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    Serruya, R., Orlovetskie, N., Reiner, R., Dehtiar-Zilber, Y., Wesolowski, D., Altman, S., & Jarrous, N. (2015). Human RNase P ribonucleoprotein is required for formation of initiation complexes of RNA polymerase III. Nucleic acids research, 43(11), 5442-5450. https://doi.org/10.1093/nar/gkv447