Human RNase P ribonucleoprotein is required for formation of initiation complexes of RNA polymerase III

Raphael Serruya, Natalie Orlovetskie, Robert Reiner, Yana Dehtiar-Zilber, Donna Wesolowski, Sidney Altman, Nayef Jarrous

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Human RNase P is implicated in transcription of small non-coding RNA genes by RNA polymerase III (Pol III), but the precise role of this ribonucleoprotein therein remains unknown. We here show that targeted destruction of HeLa nuclear RNase P inhibits transcription of 5S rRNA genes in whole cell extracts, if this precedes the stage of initiation complex formation. Biochemical purification analyses further reveal that this ribonucleoprotein is recruited to 5S rRNA genes as a part of proficient initiation complexes and the activity persists at reinitiation. Knockdown of RNase P abolishes the assembly of initiation complexes by preventing the formation of the initiation sub-complex of Pol III. Our results demonstrate that the structural intactness, but not the endoribonucleolytic activity per se, of RNase P is critical for the function of Pol III in cells and in extracts.

Original languageEnglish (US)
Pages (from-to)5442-5450
Number of pages9
JournalNucleic Acids Research
Volume43
Issue number11
DOIs
StatePublished - Apr 24 2015
Externally publishedYes

Fingerprint

Ribonuclease P
RNA Polymerase III
Ribonucleoproteins
Cell Extracts
rRNA Genes
Small Untranslated RNA
Genes

ASJC Scopus subject areas

  • Genetics
  • Medicine(all)

Cite this

Serruya, R., Orlovetskie, N., Reiner, R., Dehtiar-Zilber, Y., Wesolowski, D., Altman, S., & Jarrous, N. (2015). Human RNase P ribonucleoprotein is required for formation of initiation complexes of RNA polymerase III. Nucleic Acids Research, 43(11), 5442-5450. https://doi.org/10.1093/nar/gkv447

Human RNase P ribonucleoprotein is required for formation of initiation complexes of RNA polymerase III. / Serruya, Raphael; Orlovetskie, Natalie; Reiner, Robert; Dehtiar-Zilber, Yana; Wesolowski, Donna; Altman, Sidney; Jarrous, Nayef.

In: Nucleic Acids Research, Vol. 43, No. 11, 24.04.2015, p. 5442-5450.

Research output: Contribution to journalArticle

Serruya, R, Orlovetskie, N, Reiner, R, Dehtiar-Zilber, Y, Wesolowski, D, Altman, S & Jarrous, N 2015, 'Human RNase P ribonucleoprotein is required for formation of initiation complexes of RNA polymerase III' Nucleic Acids Research, vol. 43, no. 11, pp. 5442-5450. https://doi.org/10.1093/nar/gkv447
Serruya, Raphael ; Orlovetskie, Natalie ; Reiner, Robert ; Dehtiar-Zilber, Yana ; Wesolowski, Donna ; Altman, Sidney ; Jarrous, Nayef. / Human RNase P ribonucleoprotein is required for formation of initiation complexes of RNA polymerase III. In: Nucleic Acids Research. 2015 ; Vol. 43, No. 11. pp. 5442-5450.
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