TY - JOUR
T1 - Human leukocyte antigen Class II associations in serum antimitochondrial antibodies (AMA)-positive and AMA-negative primary biliary cirrhosis
AU - Stone, Julianne
AU - Wade, Judy A.
AU - Cauch-Dudek, Karen
AU - Ng, Chang
AU - Lindor, Keith D.
AU - Heathcote, E. Jenny
PY - 2002
Y1 - 2002
N2 - Background/Aims: An association of Class II HLA-DR8 antigen is reported in patients with serum antimitochondrial antibodies (AMA)-positive primary biliary cirrhosis (PBC); no information exists as to an association with AMA-negative PBC. We compared the frequency of HLA Class II genes in AMA-positive and AMA-negative PBC patients and healthy controls. Methods: Genomic DNA was extracted from the blood of 154 AMA-positive and 26 AMA-negative Caucasian PBC patients and from 216 healthy Caucasian controls and tested for the alleles at two HLA Class II loci, DRβ1 and DQβ1. Results: Higher allele frequencies of HLA-DRβ1*08 and DQβ1*04 were found in the AMA-positive PBC patients versus controls (14.9% vs. 6.5%, odds ratio (OR)= 3.3, global P= 0.03 and 14.4% vs. 6.5%, OR = 2.6, global P = 0.002). All patients positive for DRβ1*0801 were positive for the DQβ1*0402 allele, delta score = 22 for AMA-positive patients, 11 for controls. In AMA-negative PBC, the frequency of DRβ1*08 and DQβ1*04 was 0%, significantly different from the AMA-positive patients (P = 0.05, P: 0.05). Conclusions: AMA response may identify a group of PBC patients with a distinctive expression of the disease with the response associated with a gene(s) in the class II region of the major histocompatibility complex on the short arm of chromosome 6.
AB - Background/Aims: An association of Class II HLA-DR8 antigen is reported in patients with serum antimitochondrial antibodies (AMA)-positive primary biliary cirrhosis (PBC); no information exists as to an association with AMA-negative PBC. We compared the frequency of HLA Class II genes in AMA-positive and AMA-negative PBC patients and healthy controls. Methods: Genomic DNA was extracted from the blood of 154 AMA-positive and 26 AMA-negative Caucasian PBC patients and from 216 healthy Caucasian controls and tested for the alleles at two HLA Class II loci, DRβ1 and DQβ1. Results: Higher allele frequencies of HLA-DRβ1*08 and DQβ1*04 were found in the AMA-positive PBC patients versus controls (14.9% vs. 6.5%, odds ratio (OR)= 3.3, global P= 0.03 and 14.4% vs. 6.5%, OR = 2.6, global P = 0.002). All patients positive for DRβ1*0801 were positive for the DQβ1*0402 allele, delta score = 22 for AMA-positive patients, 11 for controls. In AMA-negative PBC, the frequency of DRβ1*08 and DQβ1*04 was 0%, significantly different from the AMA-positive patients (P = 0.05, P: 0.05). Conclusions: AMA response may identify a group of PBC patients with a distinctive expression of the disease with the response associated with a gene(s) in the class II region of the major histocompatibility complex on the short arm of chromosome 6.
KW - HLA system
KW - Primary biliary cirrhosis
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U2 - 10.1016/S0168-8278(01)00237-9
DO - 10.1016/S0168-8278(01)00237-9
M3 - Article
C2 - 11804658
AN - SCOPUS:0036128110
SN - 0168-8278
VL - 36
SP - 8
EP - 13
JO - Journal of Hepatology
JF - Journal of Hepatology
IS - 1
ER -