TY - JOUR
T1 - Hollow cone illumination for fast TEM, and outrunning damage with electrons
AU - Spence, John
AU - Subramanian, G.
AU - Musumeci, P.
N1 - Publisher Copyright:
© 2015 IOP Publishing Ltd.
PY - 2015/9/23
Y1 - 2015/9/23
N2 - We consider the possibility of imaging individual bioparticles using snapshot diffraction from femotsecond pulses, using a 3 MeV electron beam, based on the recent experimental performance of these coherent beams. Assuming that radiation damage can be outrun using 100 fs pulses (or less), we find that a sufficient number of electrons are scattered per particle only if the beam diameter can be matched to that of the particle (e.g. a virus), about three orders of magnitude smaller than has currently been demonstrated (and limited by space-charge effects). We then propose the use of the hollow-cone illumination mode for fast transmission electron microscope imaging, because it can provide full-field atomic resolution imaging despite the use of the large incoherent annular source required for an efficient photocathode, so that coherent illumination is not needed for high-resolution imaging. Reciprocity arguments are used to compare this full-field mode with data aquisition times and source brightness in scanning transmission electron microscopy.
AB - We consider the possibility of imaging individual bioparticles using snapshot diffraction from femotsecond pulses, using a 3 MeV electron beam, based on the recent experimental performance of these coherent beams. Assuming that radiation damage can be outrun using 100 fs pulses (or less), we find that a sufficient number of electrons are scattered per particle only if the beam diameter can be matched to that of the particle (e.g. a virus), about three orders of magnitude smaller than has currently been demonstrated (and limited by space-charge effects). We then propose the use of the hollow-cone illumination mode for fast transmission electron microscope imaging, because it can provide full-field atomic resolution imaging despite the use of the large incoherent annular source required for an efficient photocathode, so that coherent illumination is not needed for high-resolution imaging. Reciprocity arguments are used to compare this full-field mode with data aquisition times and source brightness in scanning transmission electron microscopy.
KW - fast imaging
KW - femtosecond electron beams
KW - radiation damage
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U2 - 10.1088/0953-4075/48/21/214003
DO - 10.1088/0953-4075/48/21/214003
M3 - Article
AN - SCOPUS:84947483921
SN - 0953-4075
VL - 48
JO - Journal of Physics B: Atomic, Molecular and Optical Physics
JF - Journal of Physics B: Atomic, Molecular and Optical Physics
IS - 21
M1 - 214003
ER -