Haploid deletion strains of Saccharomyces cerevisiae that determine survival during space flight

Kelly Johanson, Patricia L. Allen, Romer A. Gonzalez-Villalobos, Jacqueline Nesbit, Cheryl Nickerson, Kerstin Höner zu Bentrup, James W. Wilson, Rajee Ramamurthy, Riccardo D'Elia, Kenneth E. Muse, Jeffrey Hammond, Jake Freeman, Louis S. Stodieck, Timothy G. Hammond

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

This study identifies genes that determine survival during a space flight, using the model eukaryotic organism, Saccharomyces cerevisiae. Select strains of a haploid yeast deletion series grew during storage in distilled water in space, but not in ground based static or clinorotation controls. The survival advantages in space in distilled water include a 133-fold advantage for the deletion of PEX19, a chaperone and import receptor for newly- synthesized class I peroxisomal membrane proteins, to 77-40 fold for deletion strains lacking elements of aerobic respiration, isocitrate metabolism, and mitochondrial electron transport. Following automated addition of rich growth media, the space flight was associated with a marked survival advantage of strains with deletions in catalytically active genes including hydrolases, oxidoreductases and transferases. When compared to static controls, space flight was associated with a marked survival disadvantage of deletion strains lacking transporter, antioxidant and catalytic activity. This study identifies yeast deletion strains with a survival advantage during storage in distilled water and space flight, and amplifies our understanding of the genes critical for survival in space.

Original languageEnglish (US)
Pages (from-to)460-471
Number of pages12
JournalActa Astronautica
Volume60
Issue number4-7 SPEC. ISS.
DOIs
StatePublished - Feb 2007

Fingerprint

Space flight
Yeast
Genes
Hydrolases
Water
Antioxidants
Metabolism
Catalyst activity
Proteins
Membranes

Keywords

  • Deletion series
  • Space flight
  • Suspension culture
  • Yeast

ASJC Scopus subject areas

  • Aerospace Engineering

Cite this

Johanson, K., L. Allen, P., Gonzalez-Villalobos, R. A., Nesbit, J., Nickerson, C., Höner zu Bentrup, K., ... Hammond, T. G. (2007). Haploid deletion strains of Saccharomyces cerevisiae that determine survival during space flight. Acta Astronautica, 60(4-7 SPEC. ISS.), 460-471. https://doi.org/10.1016/j.actaastro.2006.09.011

Haploid deletion strains of Saccharomyces cerevisiae that determine survival during space flight. / Johanson, Kelly; L. Allen, Patricia; Gonzalez-Villalobos, Romer A.; Nesbit, Jacqueline; Nickerson, Cheryl; Höner zu Bentrup, Kerstin; Wilson, James W.; Ramamurthy, Rajee; D'Elia, Riccardo; Muse, Kenneth E.; Hammond, Jeffrey; Freeman, Jake; Stodieck, Louis S.; Hammond, Timothy G.

In: Acta Astronautica, Vol. 60, No. 4-7 SPEC. ISS., 02.2007, p. 460-471.

Research output: Contribution to journalArticle

Johanson, K, L. Allen, P, Gonzalez-Villalobos, RA, Nesbit, J, Nickerson, C, Höner zu Bentrup, K, Wilson, JW, Ramamurthy, R, D'Elia, R, Muse, KE, Hammond, J, Freeman, J, Stodieck, LS & Hammond, TG 2007, 'Haploid deletion strains of Saccharomyces cerevisiae that determine survival during space flight', Acta Astronautica, vol. 60, no. 4-7 SPEC. ISS., pp. 460-471. https://doi.org/10.1016/j.actaastro.2006.09.011
Johanson K, L. Allen P, Gonzalez-Villalobos RA, Nesbit J, Nickerson C, Höner zu Bentrup K et al. Haploid deletion strains of Saccharomyces cerevisiae that determine survival during space flight. Acta Astronautica. 2007 Feb;60(4-7 SPEC. ISS.):460-471. https://doi.org/10.1016/j.actaastro.2006.09.011
Johanson, Kelly ; L. Allen, Patricia ; Gonzalez-Villalobos, Romer A. ; Nesbit, Jacqueline ; Nickerson, Cheryl ; Höner zu Bentrup, Kerstin ; Wilson, James W. ; Ramamurthy, Rajee ; D'Elia, Riccardo ; Muse, Kenneth E. ; Hammond, Jeffrey ; Freeman, Jake ; Stodieck, Louis S. ; Hammond, Timothy G. / Haploid deletion strains of Saccharomyces cerevisiae that determine survival during space flight. In: Acta Astronautica. 2007 ; Vol. 60, No. 4-7 SPEC. ISS. pp. 460-471.
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