Genome-wide detection of polymorphisms at nucleotide resolution with a single DNA Microarray

David Gresham, Douglas M. Ruderfer, Stephen Pratt, Joseph Schacherer, Maitreya J. Dunham, David Botstein, Leonid Kruglyak

Research output: Contribution to journalArticle

216 Citations (Scopus)

Abstract

A central challenge of genomics is to detect, simply and inexpensively, all differences in sequence among the genomes of individual members of a species. We devised a system to detect all single-nucleotide differences between genomes with the use of data from a single hybridization to a whole-genome DNA microarray. This allowed us to detect a variety of spontaneous single-base pair substitutions, insertions, and deletions, and most (>90%) of the ∼30,000 known single-nucleotide polymorphisms between two Saccharomyces cerevisiae strains. We applied this approach to elucidate the genetic basis of phenotypic variants and to identify the small number of single-base pair changes accumulated during experimental evolution of yeast.

Original languageEnglish (US)
Pages (from-to)1932-1936
Number of pages5
JournalScience
Volume311
Issue number5769
DOIs
StatePublished - Mar 31 2006
Externally publishedYes

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Oligonucleotide Array Sequence Analysis
Nucleotides
Genome
Base Pairing
Genomics
Single Nucleotide Polymorphism
Saccharomyces cerevisiae
Yeasts

ASJC Scopus subject areas

  • General

Cite this

Gresham, D., Ruderfer, D. M., Pratt, S., Schacherer, J., Dunham, M. J., Botstein, D., & Kruglyak, L. (2006). Genome-wide detection of polymorphisms at nucleotide resolution with a single DNA Microarray. Science, 311(5769), 1932-1936. https://doi.org/10.1126/science.1123726

Genome-wide detection of polymorphisms at nucleotide resolution with a single DNA Microarray. / Gresham, David; Ruderfer, Douglas M.; Pratt, Stephen; Schacherer, Joseph; Dunham, Maitreya J.; Botstein, David; Kruglyak, Leonid.

In: Science, Vol. 311, No. 5769, 31.03.2006, p. 1932-1936.

Research output: Contribution to journalArticle

Gresham, D, Ruderfer, DM, Pratt, S, Schacherer, J, Dunham, MJ, Botstein, D & Kruglyak, L 2006, 'Genome-wide detection of polymorphisms at nucleotide resolution with a single DNA Microarray', Science, vol. 311, no. 5769, pp. 1932-1936. https://doi.org/10.1126/science.1123726
Gresham D, Ruderfer DM, Pratt S, Schacherer J, Dunham MJ, Botstein D et al. Genome-wide detection of polymorphisms at nucleotide resolution with a single DNA Microarray. Science. 2006 Mar 31;311(5769):1932-1936. https://doi.org/10.1126/science.1123726
Gresham, David ; Ruderfer, Douglas M. ; Pratt, Stephen ; Schacherer, Joseph ; Dunham, Maitreya J. ; Botstein, David ; Kruglyak, Leonid. / Genome-wide detection of polymorphisms at nucleotide resolution with a single DNA Microarray. In: Science. 2006 ; Vol. 311, No. 5769. pp. 1932-1936.
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