Genome-wide detection of polymorphisms at nucleotide resolution with a single DNA Microarray

David Gresham, Douglas M. Ruderfer, Stephen C. Pratt, Joseph Schacherer, Maitreya J. Dunham, David Botstein, Leonid Kruglyak

Research output: Contribution to journalArticle

220 Scopus citations

Abstract

A central challenge of genomics is to detect, simply and inexpensively, all differences in sequence among the genomes of individual members of a species. We devised a system to detect all single-nucleotide differences between genomes with the use of data from a single hybridization to a whole-genome DNA microarray. This allowed us to detect a variety of spontaneous single-base pair substitutions, insertions, and deletions, and most (>90%) of the ∼30,000 known single-nucleotide polymorphisms between two Saccharomyces cerevisiae strains. We applied this approach to elucidate the genetic basis of phenotypic variants and to identify the small number of single-base pair changes accumulated during experimental evolution of yeast.

Original languageEnglish (US)
Pages (from-to)1932-1936
Number of pages5
JournalScience
Volume311
Issue number5769
DOIs
StatePublished - Mar 31 2006
Externally publishedYes

ASJC Scopus subject areas

  • General

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    Gresham, D., Ruderfer, D. M., Pratt, S. C., Schacherer, J., Dunham, M. J., Botstein, D., & Kruglyak, L. (2006). Genome-wide detection of polymorphisms at nucleotide resolution with a single DNA Microarray. Science, 311(5769), 1932-1936. https://doi.org/10.1126/science.1123726