TY - JOUR
T1 - Genome-wide analysis of transcriptional changes in the thoracic muscle of the migratory locust, Locusta migratoria, exposed to hypobaric hypoxia
AU - Zhao, De Jian
AU - Zhang, Zhen Yu
AU - Harrison, Jon
AU - Kang, Le
N1 - Funding Information:
We would like to thank Drs. Jianing Wei, Bing Chen, Xianhui Wang, Feng Cui, Zongyuan Ma, Wei Guo, Sufang Zhang and Feng Jiang from Institute of Zoology, Chinese Academy of Sciences, and Dr. Arianne Cease from Arizona State University, for their critical review of the draft manuscript. This research was supported by Grants from National Natural Science Foundation of China (Nos. 31070366 and 30970407 ) and National Basic Research Program of China (No. 2012CB114102 ).
PY - 2012/11
Y1 - 2012/11
N2 - Hypobaric hypoxia has both beneficial and detrimental effects on living organisms in high altitude regions. The impact of hypobaric hypoxia has been investigated in numerous vertebrates. However, it is still not well characterized how invertebrates respond to hypobaric hypoxia. In this study, we examined the transcriptional profiles of locust thoracic muscles using microarrays to disclose their strategies to cope with hypobaric hypoxia. We found that hypoxia-inducible factor (HIF) and its target genes did not respond significantly to hypobaric hypoxia. As with severe, normobaric hypoxia, mitochondrial activities were systemically suppressed, mainly involving in energy production and mitochondrial biogenesis. The surveillance processes, involving in clearance of dysfunctional proteins in endoplasmic reticulum, were activated, e.g. endoplasmic reticulum-associated degradation, protein glycosylation, and protein folding. In contrast to severe, normobaric hypoxia, glycolysis was suppressed and the pentose phosphate pathway strengthened. Our data suggested that hypobaric hypoxia induced an oxidative stress rather than an energy crisis in locust thoracic muscles. Our research provides a different perspective of biological responses to hypoxia, complementing the well-studied biological responses to extreme, normobaric hypoxia.
AB - Hypobaric hypoxia has both beneficial and detrimental effects on living organisms in high altitude regions. The impact of hypobaric hypoxia has been investigated in numerous vertebrates. However, it is still not well characterized how invertebrates respond to hypobaric hypoxia. In this study, we examined the transcriptional profiles of locust thoracic muscles using microarrays to disclose their strategies to cope with hypobaric hypoxia. We found that hypoxia-inducible factor (HIF) and its target genes did not respond significantly to hypobaric hypoxia. As with severe, normobaric hypoxia, mitochondrial activities were systemically suppressed, mainly involving in energy production and mitochondrial biogenesis. The surveillance processes, involving in clearance of dysfunctional proteins in endoplasmic reticulum, were activated, e.g. endoplasmic reticulum-associated degradation, protein glycosylation, and protein folding. In contrast to severe, normobaric hypoxia, glycolysis was suppressed and the pentose phosphate pathway strengthened. Our data suggested that hypobaric hypoxia induced an oxidative stress rather than an energy crisis in locust thoracic muscles. Our research provides a different perspective of biological responses to hypoxia, complementing the well-studied biological responses to extreme, normobaric hypoxia.
KW - Altitude hypoxia
KW - Gene expression
KW - Hypobaric hypoxia
KW - Locusta migratoria
KW - Microarray
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U2 - 10.1016/j.jinsphys.2012.08.006
DO - 10.1016/j.jinsphys.2012.08.006
M3 - Article
C2 - 22985864
AN - SCOPUS:84867848169
SN - 0022-1910
VL - 58
SP - 1424
EP - 1431
JO - Journal of insect physiology
JF - Journal of insect physiology
IS - 11
ER -