@article{1d9bc13b2be644219e5ace847184005a,
title = "Genetic characterization of a recombinant myxoma virus in the iberian hare (Lepus granatensis)",
abstract = "Myxomatosis is a lethal disease in wild European and domestic rabbits (Oryctolagus cuniculus), which is caused by a Myxoma virus (MYXV) infection—a leporipoxvirus that is found naturally in some Sylvilagus rabbit species in South America and California. The introduction of MYXV into feral European rabbit populations of Australia and Europe, in the early 1950s, demonstrated the best-documented field example of host–virus coevolution, following a cross-species transmission. Recently, a new cross-species jump of MYXV has been suggested in both Great Britain and Spain, where European brown hares (Lepus europaeus) and Iberian hares (Lepus granatensis) were found dead with lesions consistent with those observed in myxomatosis. To investigate the possibility of a new cross-species transmission event by MYXV, tissue samples collected from a wild Iberian hare found dead in Spain (Toledo region) were analyzed and deep sequenced. Our results reported a new MYXV isolate (MYXV Toledo) in the tissues of this species. The genome of this new virus was found to encode three disruptive genes (M009L, M036L, and M152R) and a novel ~2.8 kb recombinant region, which resulted from an insertion of four novel poxviral genes towards the 3{\textquoteright} end of the negative strand of its genome. From the open reading frames inserted into the MYXV Toledo virus, a new orthologue of a poxvirus host range gene family member was identified, which was related to the MYXV gene M064R. Overall, we confirmed the identity of a new MYXV isolate in Iberian hares, which, we hypothesized, was able to more effectively counteract the host defenses in hares and start an infectious process in this new host.",
keywords = "Lepus granatensis, Myxoma virus, Poxvirus, Recombinant virus",
author = "Ana {\'A}gueda-Pinto and Matos, {Ana Lemos de} and M{\'a}rio Abrantes and Simona Kraberger and Risalde, {Maria A.} and Christian Gort{\'a}zar and Grant McFadden and Arvind Varsani and Esteves, {Pedro J.}",
note = "Funding Information: Funding: All animal sampling took place post-mortem. According to EU and National legislation (2010/63/UE Directive, the Spanish Royal Decree (53/2013), and the University of Castilla–La Mancha guidelines, no permission or consent was required to conduct the research reported herein. FCT—Foundation for Science and Technology supported the doctoral fellowship of A.A.-P. (ref. SFRH/BD/128752/2017) and the investigator grant of P.J.E. (IF/00376/2015). This article is a result of the project NORTE-01-0145-FEDER-000007, supported by Norte Portugal Regional Operational Programme (NORTE2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF). This work was also supported by the National Institute of Health (NIH) grant R01 A1080607. Funding Information: All animal sampling took place post-mortem. According to EU and National legislation (2010/63/UE Directive, the Spanish Royal Decree (53/2013), and the University of Castilla–La Mancha guidelines, no permission or consent was required to conduct the research reported herein. FCT—Foundation for Science and Technology supported the doctoral fellowship of A.A.-P. (ref. SFRH/BD/128752/2017) and the investigator grant of P.J.E. (IF/00376/2015). This article is a result of the project NORTE-01-0145-FEDER-000007, supported by Norte Portugal Regional Operational Programme (NORTE2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF). This work was also supported by the National Institute of Health (NIH) grant R01 A1080607. Publisher Copyright: {\textcopyright} 2019 by the authors. Licensee MDPI, Basel, Switzerland.",
year = "2019",
month = jun,
doi = "10.3390/v11060530",
language = "English (US)",
volume = "11",
journal = "Viruses",
issn = "1999-4915",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "6",
}