Fungal communities of lichen-dominated biological soil crusts: Diversity, relative microbial biomass, and their relationship to disturbance and crust cover

Scott T. Bates, Thomas H. Nash, Ken Sweat, Ferran Garcia-Pichel

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    53 Citations (Scopus)

    Abstract

    Molecular methodologies were used to characterize fungal communities associated with lichen-dominated biological soil crusts (BSCs) at two sites on the Colorado Plateau (USA) in order to investigate their diversity and abundance, in relation to that of bacteria, as well as how these parameters corresponded to overall soil crust cover and the presence of anthropogenic disturbance. Fungal community diversity and composition were assessed with denaturing gradient gel electrophoresis (DGGE) fingerprinting of PCR amplified ribosomal genes and by sequencing. Quantitative PCR, specific for fungi as well as bacteria, was used to evaluate relative microbial densities. Two sites with similar soil characteristics, both of which contained well developed BSCs dominated by lichens, were studied. Results indicated that while a considerable diversity of fungi is present within these BSCs, much higher than what has previously been determined for cyanobacteria-dominated crusts, fungi contribute less biomass and are less diverse than their bacterial counterparts. Fungal diversity in lichen-dominated BSCs was negatively correlated with disturbance and positively correlated with crust cover.

    Original languageEnglish (US)
    Pages (from-to)1192-1199
    Number of pages8
    JournalJournal of Arid Environments
    Volume74
    Issue number10
    DOIs
    StatePublished - Oct 2010

    Fingerprint

    soil crusts
    soil crust
    fungal communities
    lichen
    lichens
    microbial biomass
    crust
    disturbance
    biomass
    fungus
    fungi
    bacterium
    bacteria
    denaturing gradient gel electrophoresis
    anthropogenic activities
    Cyanobacteria
    cyanobacterium
    electrokinesis
    plateaus
    quantitative polymerase chain reaction

    Keywords

    • Bacteria
    • Biological soil crust
    • Colorado Plateau
    • DGGE
    • Fungal diversity
    • Lichens

    ASJC Scopus subject areas

    • Ecology, Evolution, Behavior and Systematics
    • Earth-Surface Processes
    • Ecology

    Cite this

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    title = "Fungal communities of lichen-dominated biological soil crusts: Diversity, relative microbial biomass, and their relationship to disturbance and crust cover",
    abstract = "Molecular methodologies were used to characterize fungal communities associated with lichen-dominated biological soil crusts (BSCs) at two sites on the Colorado Plateau (USA) in order to investigate their diversity and abundance, in relation to that of bacteria, as well as how these parameters corresponded to overall soil crust cover and the presence of anthropogenic disturbance. Fungal community diversity and composition were assessed with denaturing gradient gel electrophoresis (DGGE) fingerprinting of PCR amplified ribosomal genes and by sequencing. Quantitative PCR, specific for fungi as well as bacteria, was used to evaluate relative microbial densities. Two sites with similar soil characteristics, both of which contained well developed BSCs dominated by lichens, were studied. Results indicated that while a considerable diversity of fungi is present within these BSCs, much higher than what has previously been determined for cyanobacteria-dominated crusts, fungi contribute less biomass and are less diverse than their bacterial counterparts. Fungal diversity in lichen-dominated BSCs was negatively correlated with disturbance and positively correlated with crust cover.",
    keywords = "Bacteria, Biological soil crust, Colorado Plateau, DGGE, Fungal diversity, Lichens",
    author = "Bates, {Scott T.} and Nash, {Thomas H.} and Ken Sweat and Ferran Garcia-Pichel",
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    T2 - Diversity, relative microbial biomass, and their relationship to disturbance and crust cover

    AU - Bates, Scott T.

    AU - Nash, Thomas H.

    AU - Sweat, Ken

    AU - Garcia-Pichel, Ferran

    PY - 2010/10

    Y1 - 2010/10

    N2 - Molecular methodologies were used to characterize fungal communities associated with lichen-dominated biological soil crusts (BSCs) at two sites on the Colorado Plateau (USA) in order to investigate their diversity and abundance, in relation to that of bacteria, as well as how these parameters corresponded to overall soil crust cover and the presence of anthropogenic disturbance. Fungal community diversity and composition were assessed with denaturing gradient gel electrophoresis (DGGE) fingerprinting of PCR amplified ribosomal genes and by sequencing. Quantitative PCR, specific for fungi as well as bacteria, was used to evaluate relative microbial densities. Two sites with similar soil characteristics, both of which contained well developed BSCs dominated by lichens, were studied. Results indicated that while a considerable diversity of fungi is present within these BSCs, much higher than what has previously been determined for cyanobacteria-dominated crusts, fungi contribute less biomass and are less diverse than their bacterial counterparts. Fungal diversity in lichen-dominated BSCs was negatively correlated with disturbance and positively correlated with crust cover.

    AB - Molecular methodologies were used to characterize fungal communities associated with lichen-dominated biological soil crusts (BSCs) at two sites on the Colorado Plateau (USA) in order to investigate their diversity and abundance, in relation to that of bacteria, as well as how these parameters corresponded to overall soil crust cover and the presence of anthropogenic disturbance. Fungal community diversity and composition were assessed with denaturing gradient gel electrophoresis (DGGE) fingerprinting of PCR amplified ribosomal genes and by sequencing. Quantitative PCR, specific for fungi as well as bacteria, was used to evaluate relative microbial densities. Two sites with similar soil characteristics, both of which contained well developed BSCs dominated by lichens, were studied. Results indicated that while a considerable diversity of fungi is present within these BSCs, much higher than what has previously been determined for cyanobacteria-dominated crusts, fungi contribute less biomass and are less diverse than their bacterial counterparts. Fungal diversity in lichen-dominated BSCs was negatively correlated with disturbance and positively correlated with crust cover.

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    KW - DGGE

    KW - Fungal diversity

    KW - Lichens

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