An analysis of the use-stage for pavement Life-Cycle Assessment (LCA) is presented within a framework of an LCA tool developed for Illinois Tollway. Methodological choices that can significantly affect LCA results were evaluated in this study. The share of the use-stage in a comprehensive pavement LCA framework was evaluated with all life-cycle stages including materials, construction, use, maintenance, and end of life. The scope of the use-stage includes albedo, carbonation, and rolling resistance (including pavement roughness and texture components). Uncertainty of the pavement roughness and its effect on the results are investigated using Monte Carlo sampling technique. A discussion on the choice of functional unit and its effect in comparative LCA is presented and an appropriate functional unit is suggested. Four projects were selected as case studies to demonstrate the capabilities of the tool and proposed framework. A multi-point environmental performance evaluation was performed using four environmental indicators for comprehensive interpretation of results. The effect of each stage of LCA was evaluated with emphasis on the results of the use-stage. Additional fuel consumption and emissions, resulting from roughness and texture, constituted the largest share of use-stage impacts while the effect of carbonation was limited. As traffic reduced and the share of the materials and construction stage increased, the share of the use-stage could be decreased to 50% levels.