Fluorescent Protein approaches in alpha Herpesvirus Research

Ian Hogue, Jens B. Bosse, Esteban A. Engel, Julian Scherer, Jiun Ruey Hu, Tony Del Rio, Lynn W. Enquist

Research output: Contribution to journalReview article

19 Scopus citations

Abstract

In the nearly two decades since the popularization of green fluorescent protein (GFP), fluorescent protein-based methodologies have revolutionized molecular and cell biology, allowing us to literally see biological processes as never before. Naturally, this revolution has extended to virology in general, and to the study of alpha herpesviruses in particular. In this review, we provide a compendium of reported fluorescent protein fusions to herpes simplex virus 1 (HSV-1) and pseudorabies virus (PRV) structural proteins, discuss the underappreciated challenges of fluorescent protein-based approaches in the context of a replicating virus, and describe general strategies and best practices for creating new fluorescent fusions. We compare fluorescent protein methods to alternative approaches, and review two instructive examples of the caveats associated with fluorescent protein fusions, including describing several improved fluorescent capsid fusions in PRV. Finally, we present our future perspectives on the types of powerful experiments these tools now offer.

Original languageEnglish (US)
Pages (from-to)5933-5961
Number of pages29
JournalViruses
Volume7
Issue number11
DOIs
StatePublished - Nov 19 2015
Externally publishedYes

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Keywords

  • Alpha herpesvirus
  • Capsid
  • Fluorescence microscopy
  • Fluorescent protein
  • GFP
  • Herpes simplex virus
  • HSV
  • Pseudorabies virus

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology

Cite this

Hogue, I., Bosse, J. B., Engel, E. A., Scherer, J., Hu, J. R., Del Rio, T., & Enquist, L. W. (2015). Fluorescent Protein approaches in alpha Herpesvirus Research. Viruses, 7(11), 5933-5961. https://doi.org/10.3390/v7112915