Fluorescence spectroscopic analysis of circulating platelet activation during coronary angioplasty

Alexander Christov, William J. Kostuk, George Jablonsky, Alexandra Lucas

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Background and Objective: Platelet activation during percutaneous transluminal coronary angioplasty (PTCA) initiates thrombus formation and plaque regrowth at sites of arterial injury, limiting procedure efficacy. We have developed a simple assay for circulating platelet activation based on fluorescence analysis of membrane fluidity and intracellular calcium concentration and light scattering analysis of platelet aggregation. Study Design/Materials and Methods: Platelet activation state was measured in 45 patients undergoing angioplasty, before and after treatment with platelet inhibitors. Results: PTCA alone produced a decrease in pyrene dimer formation (P ≤ 0.0083) and an increase in light scattering at 650 nm (P ≤ 0.0128). Treatment with ADP and GPIIb/IIIa receptor antagonists reduced PTCA induced changes in pyrene dimer formation. An unexpected decrease in pyrene dimer formation (P ≤ 0.05) was detected when the GPIIb/IIIa receptor antagonist was given together with an ADP receptor antagonist. Conclusions: 1) Analysis of membrane fluidity provides a sensitive marker for platelet activation state. 2) Reduced membrane fluidity after combined platelet inhibitor treatments suggests reduced antiplatelet efficacy.

Original languageEnglish (US)
Pages (from-to)414-426
Number of pages13
JournalLasers in Surgery and Medicine
Volume28
Issue number5
DOIs
StatePublished - 2001
Externally publishedYes

Keywords

  • ADP receptor antagonist
  • Coronary angioplasty
  • Fluorescence
  • GP IIb/IIIa receptor antagonist
  • Membrane fluidity
  • Platelet

ASJC Scopus subject areas

  • Surgery
  • Dermatology

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