Femtosecond structural dynamics drives the trans/cis isomerization in photoactive yellow protein

Kanupriya Pande; Christopher D M Hutchison; Gerrit Groenhof; Andy Aquila; Josef S. Robinson; Jason Tenboer; Shibom Basu; Sébastien Boutet; Daniel P. DePonte; Mengning Liang; Thomas A. White; Nadia Zatsepin; Oleksandr Yefanov; Dmitry Morozov; Dominik Oberthuer; Cornelius Gati; Ganesh Subramanian; Daniel James; Yun Zhao; Jake Koralek; Jennifer Brayshaw; Christopher Kupitz; Chelsie Conrad; Shatabdi Roy-Chowdhury; Jesse D. Coe; Markus Metz; Paulraj Lourdu Xavier; Thomas D. Grant; Jason E. Koglin; Gihan Ketawala; Raimund Fromme; Vukica Srajer; Robert Henning; John Spence; Abbas Ourmazd; Peter Schwander; Uwe Weierstall; Matthias Frank; Petra Fromme; Anton Barty; Henry N. Chapman; Keith Moffat; Jasper J. Van Thor; Marius Schmidt

doi:10.1126/science.aad5081

Femtosecond structural dynamics drives the trans/cis isomerization in photoactive yellow protein

Kanupriya Pande, Christopher D M Hutchison, Gerrit Groenhof, Andy Aquila, Josef S. Robinson, Jason Tenboer, Shibom Basu, Sébastien Boutet, Daniel P. DePonte, Mengning Liang, Thomas A. White, Nadia Zatsepin, Oleksandr Yefanov, Dmitry Morozov, Dominik Oberthuer, Cornelius Gati, Ganesh Subramanian, Daniel James, Yun Zhao, Jake KoralekJennifer Brayshaw, Christopher Kupitz, Chelsie Conrad, Shatabdi Roy-Chowdhury, Jesse D. Coe, Markus Metz, Paulraj Lourdu Xavier, Thomas D. Grant, Jason E. Koglin, Gihan Ketawala, Raimund Fromme, Vukica Srajer, Robert Henning, John Spence, Abbas Ourmazd, Peter Schwander, Uwe Weierstall, Matthias Frank, Petra Fromme, Anton Barty, Henry N. Chapman, Keith Moffat, Jasper J. Van Thor, Marius Schmidt

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Abstract

A variety of organisms have evolved mechanisms to detect and respond to light, in which the response is mediated by protein structural changes after photon absorption. The initial step is often the photoisomerization of a conjugated chromophore. Isomerization occurs on ultrafast time scales and is substantially influenced by the chromophore environment. Here we identify structural changes associated with the earliest steps in the trans-to-cis isomerization of the chromophore in photoactive yellow protein. Femtosecond hard x-ray pulses emitted by the Linac Coherent Light Source were used to conduct time-resolved serial femtosecond crystallography on photoactive yellow protein microcrystals over a time range from 100 femtoseconds to 3 picoseconds to determine the structural dynamics of the photoisomerization reaction.

Original language	English (US)
Pages (from-to)	725-729
Number of pages	5
Journal	Science
Volume	352
Issue number	6286
DOIs	https://doi.org/10.1126/science.aad5081
State	Published - May 6 2016

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Pande, K., Hutchison, C. D. M., Groenhof, G., Aquila, A., Robinson, J. S., Tenboer, J., Basu, S., Boutet, S., DePonte, D. P., Liang, M., White, T. A., Zatsepin, N., Yefanov, O., Morozov, D., Oberthuer, D., Gati, C., Subramanian, G., James, D., Zhao, Y., ... Schmidt, M. (2016). Femtosecond structural dynamics drives the trans/cis isomerization in photoactive yellow protein. Science, 352(6286), 725-729. https://doi.org/10.1126/science.aad5081

Pande, K, Hutchison, CDM, Groenhof, G, Aquila, A, Robinson, JS, Tenboer, J, Basu, S, Boutet, S, DePonte, DP, Liang, M, White, TA, Zatsepin, N, Yefanov, O, Morozov, D, Oberthuer, D, Gati, C, Subramanian, G, James, D, Zhao, Y, Koralek, J, Brayshaw, J, Kupitz, C, Conrad, C, Roy-Chowdhury, S, Coe, JD, Metz, M, Xavier, PL, Grant, TD, Koglin, JE, Ketawala, G, Fromme, R, Srajer, V, Henning, R, Spence, J, Ourmazd, A, Schwander, P, Weierstall, U, Frank, M, Fromme, P, Barty, A, Chapman, HN, Moffat, K, Van Thor, JJ & Schmidt, M 2016, 'Femtosecond structural dynamics drives the trans/cis isomerization in photoactive yellow protein', Science, vol. 352, no. 6286, pp. 725-729. https://doi.org/10.1126/science.aad5081

@article{b22491d730814dc9818a908cd01313e0,

title = "Femtosecond structural dynamics drives the trans/cis isomerization in photoactive yellow protein",

abstract = "A variety of organisms have evolved mechanisms to detect and respond to light, in which the response is mediated by protein structural changes after photon absorption. The initial step is often the photoisomerization of a conjugated chromophore. Isomerization occurs on ultrafast time scales and is substantially influenced by the chromophore environment. Here we identify structural changes associated with the earliest steps in the trans-to-cis isomerization of the chromophore in photoactive yellow protein. Femtosecond hard x-ray pulses emitted by the Linac Coherent Light Source were used to conduct time-resolved serial femtosecond crystallography on photoactive yellow protein microcrystals over a time range from 100 femtoseconds to 3 picoseconds to determine the structural dynamics of the photoisomerization reaction.",

author = "Kanupriya Pande and Hutchison, {Christopher D M} and Gerrit Groenhof and Andy Aquila and Robinson, {Josef S.} and Jason Tenboer and Shibom Basu and S{\'e}bastien Boutet and DePonte, {Daniel P.} and Mengning Liang and White, {Thomas A.} and Nadia Zatsepin and Oleksandr Yefanov and Dmitry Morozov and Dominik Oberthuer and Cornelius Gati and Ganesh Subramanian and Daniel James and Yun Zhao and Jake Koralek and Jennifer Brayshaw and Christopher Kupitz and Chelsie Conrad and Shatabdi Roy-Chowdhury and Coe, {Jesse D.} and Markus Metz and Xavier, {Paulraj Lourdu} and Grant, {Thomas D.} and Koglin, {Jason E.} and Gihan Ketawala and Raimund Fromme and Vukica Srajer and Robert Henning and John Spence and Abbas Ourmazd and Peter Schwander and Uwe Weierstall and Matthias Frank and Petra Fromme and Anton Barty and Chapman, {Henry N.} and Keith Moffat and {Van Thor}, {Jasper J.} and Marius Schmidt",

note = "Funding Information: This work is supported by the NSF Science and Technology Center BioXFEL (grant NSF-1231306); by NIH grants R01GM095583 (P.F.), R01EY024363 (K.M.), and R24GM111072 (V.S., R.H., and K.M.); Helmholtz Association Virtual Institute Dynamic Pathways (H.C.); and grant NSF-0952643 (M.S.). K.P. is partly supported by grant NSF-1158138 (to D. Saldin and M.S.) and Federal Ministry of Education and Research, Germany (BMBF) grant 05K14CHA (to H.C.). J.J.v.T.",

year = "2016",

month = may,

day = "6",

doi = "10.1126/science.aad5081",

language = "English (US)",

volume = "352",

pages = "725--729",

journal = "Science",

issn = "0036-8075",

publisher = "American Association for the Advancement of Science",

number = "6286",

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TY - JOUR

T1 - Femtosecond structural dynamics drives the trans/cis isomerization in photoactive yellow protein

AU - Pande, Kanupriya

AU - Hutchison, Christopher D M

AU - Groenhof, Gerrit

AU - Aquila, Andy

AU - Robinson, Josef S.

AU - Tenboer, Jason

AU - Basu, Shibom

AU - Boutet, Sébastien

AU - DePonte, Daniel P.

AU - Liang, Mengning

AU - White, Thomas A.

AU - Zatsepin, Nadia

AU - Yefanov, Oleksandr

AU - Morozov, Dmitry

AU - Oberthuer, Dominik

AU - Gati, Cornelius

AU - Subramanian, Ganesh

AU - James, Daniel

AU - Zhao, Yun

AU - Koralek, Jake

AU - Brayshaw, Jennifer

AU - Kupitz, Christopher

AU - Conrad, Chelsie

AU - Roy-Chowdhury, Shatabdi

AU - Coe, Jesse D.

AU - Metz, Markus

AU - Xavier, Paulraj Lourdu

AU - Grant, Thomas D.

AU - Koglin, Jason E.

AU - Ketawala, Gihan

AU - Fromme, Raimund

AU - Srajer, Vukica

AU - Henning, Robert

AU - Spence, John

AU - Ourmazd, Abbas

AU - Schwander, Peter

AU - Weierstall, Uwe

AU - Frank, Matthias

AU - Fromme, Petra

AU - Barty, Anton

AU - Chapman, Henry N.

AU - Moffat, Keith

AU - Van Thor, Jasper J.

AU - Schmidt, Marius

N1 - Funding Information: This work is supported by the NSF Science and Technology Center BioXFEL (grant NSF-1231306); by NIH grants R01GM095583 (P.F.), R01EY024363 (K.M.), and R24GM111072 (V.S., R.H., and K.M.); Helmholtz Association Virtual Institute Dynamic Pathways (H.C.); and grant NSF-0952643 (M.S.). K.P. is partly supported by grant NSF-1158138 (to D. Saldin and M.S.) and Federal Ministry of Education and Research, Germany (BMBF) grant 05K14CHA (to H.C.). J.J.v.T.

PY - 2016/5/6

Y1 - 2016/5/6

N2 - A variety of organisms have evolved mechanisms to detect and respond to light, in which the response is mediated by protein structural changes after photon absorption. The initial step is often the photoisomerization of a conjugated chromophore. Isomerization occurs on ultrafast time scales and is substantially influenced by the chromophore environment. Here we identify structural changes associated with the earliest steps in the trans-to-cis isomerization of the chromophore in photoactive yellow protein. Femtosecond hard x-ray pulses emitted by the Linac Coherent Light Source were used to conduct time-resolved serial femtosecond crystallography on photoactive yellow protein microcrystals over a time range from 100 femtoseconds to 3 picoseconds to determine the structural dynamics of the photoisomerization reaction.

AB - A variety of organisms have evolved mechanisms to detect and respond to light, in which the response is mediated by protein structural changes after photon absorption. The initial step is often the photoisomerization of a conjugated chromophore. Isomerization occurs on ultrafast time scales and is substantially influenced by the chromophore environment. Here we identify structural changes associated with the earliest steps in the trans-to-cis isomerization of the chromophore in photoactive yellow protein. Femtosecond hard x-ray pulses emitted by the Linac Coherent Light Source were used to conduct time-resolved serial femtosecond crystallography on photoactive yellow protein microcrystals over a time range from 100 femtoseconds to 3 picoseconds to determine the structural dynamics of the photoisomerization reaction.

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UR - http://www.scopus.com/inward/citedby.url?scp=84966311116&partnerID=8YFLogxK

U2 - 10.1126/science.aad5081

DO - 10.1126/science.aad5081

M3 - Article

C2 - 27151871

AN - SCOPUS:84966311116

SN - 0036-8075

VL - 352

SP - 725

EP - 729

JO - Science

JF - Science

IS - 6286

ER -

Femtosecond structural dynamics drives the trans/cis isomerization in photoactive yellow protein

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