Feasibility of using an oleate-rich diet to reduce the susceptibility of low-density lipoprotein to oxidative modification in humans

Peter Reaven, Sampath Parthasarathy, Barbara J. Grasse, Elizabeth Miller, Felicidad Almazan, Fred H. Mattson, John C. Khoo, Daniel Steinberg, Joseph L. Witztum

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265 Scopus citations

Abstract

Oxidized low-density lipoprotein (LDL) is more atherogenic than native LDL. The initial step in the oxidation is the peroxidation of polyunsaturated fatty acids. Thus, decreasing the concentration of polyunsaturated fatty acids should reduce the susceptibility of LDL to oxidation. Therefore, we tested the possibility that diets enriched in oleate might result in LDL that is less susceptible to oxidative modification. LDL isolated from subjects consuming an oleate-enriched diet, compared with LDL from subjects on a linoleate-enriched diet, contained significantly more oleate (28.7% vs 11.5%) and less linoleate (31.9% vs 50.9%). Generation of conjugated dienes was significantly lower in the LDL from the oleate group. Most important, after incubation with endothelial cells, LDL from the oleate group underwent less degradation by macrophages. These studies demonstrate the feasibility of altering the diet in a way that will not raise LDL cholesterol concentrations and yet will decrease the susceptibility of LDL to oxidative modification.

Original languageEnglish (US)
Pages (from-to)701-706
Number of pages6
JournalAmerican Journal of Clinical Nutrition
Volume54
Issue number4
DOIs
StatePublished - Oct 1991

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Keywords

  • Atherosclerosis
  • Conjugated dienes
  • Macrophages

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Reaven, P., Parthasarathy, S., Grasse, B. J., Miller, E., Almazan, F., Mattson, F. H., Khoo, J. C., Steinberg, D., & Witztum, J. L. (1991). Feasibility of using an oleate-rich diet to reduce the susceptibility of low-density lipoprotein to oxidative modification in humans. American Journal of Clinical Nutrition, 54(4), 701-706. https://doi.org/10.1093/ajcn/54.4.701