Extraordinary genome stability in the ciliate Paramecium tetraurelia

Way Sung, Abraham E. Tucker, Thomas G. Doak, Eunjin Choi, W. Kelley Thomas, Michael Lynch

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Mutation plays a central role in all evolutionary processes and is also the basis of genetic disorders. Established base-substitution mutation rates in eukaryotes range between ∼5 × 10-10 and 5 × 10 -8 per site per generation, but here we report a genome-wide estimate for Paramecium tetraurelia that is more than an order of magnitude lower than any previous eukaryotic estimate. Nevertheless, when the mutation rate per cell division is extrapolated to the length of the sexual cycle for this protist, the measure obtained is comparable to that for multicellular species with similar genome sizes. Because Paramecium has a transcriptionally silent germ-line nucleus, these results are consistent with the hypothesis that natural selection operates on the cumulative germ-line replication fidelity per episode of somatic gene expression, with the germ-line mutation rate per cell division evolving downward to the lower barrier imposed by random genetic drift. We observe ciliate-specific modifications of widely conserved amino acid sites in DNA polymerases as one potential explanation for unusually high levels of replication fidelity.

Original languageEnglish (US)
Pages (from-to)19339-19344
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume109
Issue number47
DOIs
StatePublished - Nov 20 2012
Externally publishedYes

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Paramecium tetraurelia
Genomic Instability
Mutation Rate
Germ Cells
Cell Division
Paramecium
Genetic Drift
Genome Size
Inborn Genetic Diseases
Germ-Line Mutation
Genetic Selection
DNA-Directed DNA Polymerase
Eukaryota
Genome
Gene Expression
Amino Acids
Mutation

Keywords

  • Drift-barrier
  • Mutation accumulation

ASJC Scopus subject areas

  • General

Cite this

Extraordinary genome stability in the ciliate Paramecium tetraurelia. / Sung, Way; Tucker, Abraham E.; Doak, Thomas G.; Choi, Eunjin; Thomas, W. Kelley; Lynch, Michael.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, No. 47, 20.11.2012, p. 19339-19344.

Research output: Contribution to journalArticle

Sung, Way ; Tucker, Abraham E. ; Doak, Thomas G. ; Choi, Eunjin ; Thomas, W. Kelley ; Lynch, Michael. / Extraordinary genome stability in the ciliate Paramecium tetraurelia. In: Proceedings of the National Academy of Sciences of the United States of America. 2012 ; Vol. 109, No. 47. pp. 19339-19344.
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