Expression of active human tissue-type plasminogen activator in Escherichia coli

Agriculture & Biology

• t-plasminogen activator 100%
• plasminogen activator 90%
• Escherichia coli 43%
• tissues 30%
• disulfide bonds 30%
• Erythrina caffra 26%
• agarose 24%
• proteins 23%
• protein disulfide-isomerase 18%
• isomerization 16%
• bacteria 15%
• fermenters 14%
• oxidoreductases 14%
• disulfides 13%
• cysteine 11%
• lysine 11%
• resins 11%
• fermentation 9%
• yeasts 9%
• rats 7%
• enzymes 6%
• cells 4%

Medicine & Life Sciences

• Tissue Plasminogen Activator 73%
• Escherichia coli 48%
• Disulfides 43%
• Periplasm 36%
• zinc chelate-sepharose 28%
• lysine-sepharose 25%
• Erythrina 23%
• Proteins 20%
• Protein Disulfide-Isomerases 19%
• Bacteria 18%
• Fungal Proteins 17%
• Fibrinolytic Agents 16%
• Escherichia coli Proteins 16%
• Bioreactors 16%
• Fermentation 14%
• Cysteine 12%
• Oxidoreductases 12%
• Cell Count 10%
• Enzymes 7%
• In Vitro Techniques 6%

Earth & Environmental Sciences

• protein 49%
• tissue 49%
• bacterium 14%
• yeast 11%
• purification 10%
• inhibitor 10%
• fermentation 10%
• resin 10%
• enzyme 9%
• cell 7%
• need 6%
• effect 2%