Export of Extracellular Polysaccharides Modulates Adherence of the Cyanobacterium Synechocystis

Michael L. Fisher, Rebecca Allen, Yingqin Luo, Roy Curtiss

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The field of cyanobacterial biofuel production is advancing rapidly, yet we know little of the basic biology of these organisms outside of their photosynthetic pathways. We aimed to gain a greater understanding of how the cyanobacterium Synechocystis PCC 6803 (Synechocystis, hereafter) modulates its cell surface. Such understanding will allow for the creation of mutants that autoflocculate in a regulated way, thus avoiding energy intensive centrifugation in the creation of biofuels. We constructed mutant strains lacking genes predicted to function in carbohydrate transport or synthesis. Strains with gene deletions of slr0977 (predicted to encode a permease component of an ABC transporter), slr0982 (predicted to encode an ATP binding component of an ABC transporter) and slr1610 (predicted to encode a methyltransferase) demonstrated flocculent phenotypes and increased adherence to glass. Upon bioinformatic inspection, the gene products of slr0977, slr0982, and slr1610 appear to function in O-antigen (OAg) transport and synthesis. However, the analysis provided here demonstrated no differences between OAg purified from wild-type and mutants. However, exopolysaccharides (EPS) purified from mutants were altered in composition when compared to wild-type. Our data suggest that there are multiple means to modulate the cell surface of Synechocystis by disrupting different combinations of ABC transporters and/or glycosyl transferases. Further understanding of these mechanisms may allow for the development of industrially and ecologically useful strains of cyanobacteria. Additionally, these data imply that many cyanobacterial gene products may possess as-yet undiscovered functions, and are meritorious of further study.

Original languageEnglish (US)
Article numbere74514
JournalPLoS One
Volume8
Issue number9
DOIs
StatePublished - Sep 10 2013

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Synechocystis
ATP-Binding Cassette Transporters
Cyanobacteria
ABC transporters
Polysaccharides
O Antigens
polysaccharides
Biofuels
Genes
mutants
biofuels
Membrane Transport Proteins
Gene Deletion
Methyltransferases
Transferases
antigens
Computational Biology
Synechocystis sp. PCC 6803
Centrifugation
synthesis

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Export of Extracellular Polysaccharides Modulates Adherence of the Cyanobacterium Synechocystis. / Fisher, Michael L.; Allen, Rebecca; Luo, Yingqin; Curtiss, Roy.

In: PLoS One, Vol. 8, No. 9, e74514, 10.09.2013.

Research output: Contribution to journalArticle

Fisher, Michael L. ; Allen, Rebecca ; Luo, Yingqin ; Curtiss, Roy. / Export of Extracellular Polysaccharides Modulates Adherence of the Cyanobacterium Synechocystis. In: PLoS One. 2013 ; Vol. 8, No. 9.
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