Exploratory spatial analysis of in vitro respiratory syncytial virus co-infections

Ivan Simeonov, Xiaoyan Gong, Oekyung Kim, Mary Poss, Francesca Chiaromonte, John Fricks

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The cell response to virus infection and virus perturbation of that response is dynamic and is reflected by changes in cell susceptibility to infection. In this study, we evaluated the response of human epithelial cells to sequential infections with human respiratory syncytial virus strains A2 and B to determine if a primary infection with one strain will impact the ability of cells to be infected with the second as a function of virus strain and time elapsed between the two exposures. Infected cells were visualized with fluorescent markers, and location of all cells in the tissue culture well were identified using imaging software. We employed tools from spatial statistics to investigate the likelihood of a cell being infected given its proximity to a cell infected with either the homologous or heterologous virus. We used point processes, K-functions, and simulation procedures designed to account for specific features of our data when assessing spatial associations. Our results suggest that intrinsic cell properties increase susceptibility of cells to infection, more so for RSV-B than for RSV-A. Further, we provide evidence that the primary infection can decrease susceptibility of cells to the heterologous challenge virus but only at the 16 h time point evaluated in this study. Our research effort highlights the merits of integrating empirical and statistical approaches to gain greater insight on in vitro dynamics of virus-host interactions.

Original languageEnglish (US)
Pages (from-to)2782-2802
Number of pages21
JournalViruses
Volume2
Issue number12
DOIs
StatePublished - Dec 2010
Externally publishedYes

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Respiratory Syncytial Virus Infections
Spatial Analysis
Coinfection
Viruses
Infection
Human respiratory syncytial virus
In Vitro Techniques
Virus Diseases
varespladib methyl
Software
Epithelial Cells

Keywords

  • Co-infections
  • Human respiratory syncytial virus
  • Spatial statistics

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology

Cite this

Exploratory spatial analysis of in vitro respiratory syncytial virus co-infections. / Simeonov, Ivan; Gong, Xiaoyan; Kim, Oekyung; Poss, Mary; Chiaromonte, Francesca; Fricks, John.

In: Viruses, Vol. 2, No. 12, 12.2010, p. 2782-2802.

Research output: Contribution to journalArticle

Simeonov, I, Gong, X, Kim, O, Poss, M, Chiaromonte, F & Fricks, J 2010, 'Exploratory spatial analysis of in vitro respiratory syncytial virus co-infections', Viruses, vol. 2, no. 12, pp. 2782-2802. https://doi.org/10.3390/v2122782
Simeonov, Ivan ; Gong, Xiaoyan ; Kim, Oekyung ; Poss, Mary ; Chiaromonte, Francesca ; Fricks, John. / Exploratory spatial analysis of in vitro respiratory syncytial virus co-infections. In: Viruses. 2010 ; Vol. 2, No. 12. pp. 2782-2802.
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